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Your Position: Home > All Other Proteins > CD3E & CD3D > CDD-R52D8

Rat CD3 epsilon&CD3 delta Heterodimer Protein, His Tag&Flag Tag (MALS verified)

  • Synonym
    CD3E & CD3D,CD3 delta & CD3 epsilon,CD3 delta , epsilon
  • Source
    Rat CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (CDD-R52D8) is expressed from human 293 cells (HEK293). It contains AA Gln 21 - Asp 103 (CD3E) & Phe 22 - Gly 106 (CD3D) (Accession # D4A5M2-1(CD3E) & P19377-1(CD3D)).
    Predicted N-terminus: Gln 21 (CD3E) & Phe 22 (CD3D)
  • Molecular Characterization
    Online(Gln 21 - Asp 103 (CD3E) & Phe 22 - Gly 106 (CD3D)) D4A5M2-1 (CD3E) & P19377-1 (CD3D)

    Rat CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 14.9 kDa (CD3E) and 14.5 kDa (CD3D). Subunit CD3E is fused with a polyhistidine tag at the C-terminus and subunit CD3D is fused with a flag tag at the C-terminus. As a result of glycosylation, the heterodimer protein migrates as 19-20 kDa under reducing (R) condition, and 40-45 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. CDD-R52D8) SDS-PAGE gel

Rat CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Rat CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag (Cat. No. ) MALS images

The purity of Rat CD3E&CD3D Heterodimer Protein, His Tag&Flag Tag(Cat. No. CDD-R52D8) was more than 90% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.

  • Background
    T-cell surface glycoprotein CD3 delta & CD3 epsilon chain, also known as CD3D & CD3E or CD3D&CD3E respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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