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Your Position: Home > Protein > Integrin alpha X beta 2 > IT2-H52W3

Human Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer Protein, His Tag&Tag Free

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  • Synonym
    Integrin alpha X beta 2, ITGAX&ITGB2, CD11C&CD18
  • Source
    Human ITGAX&ITGB2 Heterodimer Protein, His Tag&Tag Free(IT2-H52W3) is expressed from human 293 cells (HEK293). It contains AA Phe 20 -Pro 1107 (ITGAX) & Gln 23 - Asn 700 (ITGB2) (Accession # P20702-1 (ITGAX) & P05107-1 (ITGB2)).
    Predicted N-terminus: Phe 20 (ITGAX) & Gln 23 (ITGB2)
  • Molecular Characterization
    Integrin alpha X beta 2 Structure

    Human ITGAX&ITGB2 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGAX and ITGB2, has a calculated MW of 126.5 kDa (ITGAX) and 80.2 kDa (ITGB2). Subunit ITGAX is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB2 contains no tag but a basic tail at the C-terminus. The protein migrates as 145 kDa (ITGAX) and 90 kDa (ITGB2) under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Integrin alpha X beta 2 SDS-PAGE

Human ITGAX&ITGB2 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Background
    The integrin αXβ2 takes a key role in host defense and functions as a receptor for the complement C3 cleavage product iC3b, fibrinogen, denatured proteins, and intercellular adhesion molecule (ICAM)-1. Integrin αXβ2 αXβ2 has a unique cellular distribution with expression on monocytes, macrophages, and the CD8− subsets of dendritic cells and involves in priming and regulation of the immune response.
  • Clinical and Translational Updates
  • Please contact us via if you have any question on this product.

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