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Your Position: Home > Immune Checkpoint Proteins > Nectin-2 > CD2-H82A3

Biotinylated Human Nectin-2 / CD112 Protein, Mouse IgG2a Fc,Avitag™ (MALS verified)

  • Synonym
    CD112,Nectin-2,PVRL2
  • Source
    Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (CD2-H82A3) is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Leu 360 (Accession # Q92692-2).
    Predicted N-terminus: Gln 32
  • Molecular Characterization
    Online(Gln 32 - Leu 360) Q92692-2

    This protein carries a mouse IgG2a Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 64.1 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) SDS-PAGE gel

Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) was more than 85% and the molecular weight of this protein is around 260-275 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human Nectin-2, Mouse IgG2a Fc,AvitagBiotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) ELISA bioactivity

Immobilized Human PVRIG, His Tag (Cat. No. PVG-H52H4) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) with a linear range of 0.6-10 ng/mL (QC tested).

Biotinylated Human Nectin-2, Mouse IgG2a Fc,AvitagBiotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) ELISA bioactivity

Immobilized Human DNAM-1, Fc Tag (Cat. No. DN1-H5257) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) with a linear range of 10-78 ng/mL (Routinely tested).

Biotinylated Human Nectin-2, Mouse IgG2a Fc,AvitagBiotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) ELISA bioactivity

Immobilized Biotinylated Human Nectin-2, Mouse IgG2a Fc,Avitag (Cat. No. CD2-H82A3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human PVRIG, His Tag (Cat. No. PVG-H52H4) with a linear range of 1-20 ng/mL (Routinely tested).

  • Background
    Poliovirus receptor-related 2 (PVRL2) is also known as nectin-2 and CD112 (formerly herpesvirus entry mediator B, HVEB), which is a human plasma membrane glycoprotein. PVRL2 is one of the plasma membrane components of adherens junctions. PVRL2 also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in PVRL2 gene have been associated with differences in the severity of multiple sclerosis. Also, PVRL2 binds with low affinity to TIGIT. Furthermore, PVRL2 acts as a receptor for herpes simplex virus 1 (HHV-1) mutant Rid1, herpes simplex virus 1 (HHV-2) and pseudorabies virus (PRV).
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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