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Your Position: Home > All Other Proteins > BCMA > BCA-R52H4

Rabbit BCMA / TNFRSF17 Protein,His Tag

INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    TNFRSF17,CD269,BCM,BCMA
  • Source
    Rabbit BCMA, His Tag (BCA-R52H4) is expressed from human 293 cells (HEK293). It contains AA Gln 1 - Asn 52 (Accession # G1SFN4-1).
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  • Molecular Characterization
    Online(Gln 1 - Asn 52) G1SFN4-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 7.9 kDa. The protein migrates as 12 kDa and 14 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rabbit BCMA, His Tag (Cat. No. BCA-R52H4) SDS-PAGE gel

Rabbit BCMA, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Rabbit BCMA, His TagRabbit BCMA, His Tag (Cat. No. BCA-R52H4) ELISA bioactivity

Immobilized Human APRIL, Fc Tag (Cat. No. APL-H5267) at 5 μg/mL (100 μL/well) can bind Rabbit BCMA, His Tag (Cat. No. BCA-R52H4) with a linear range of 2-5 ng/mL (QC tested).

Protocol
Rabbit BCMA, His TagRabbit BCMA, His Tag (Cat. No. BCA-R52H4) ELISA bioactivity

Immobilized Rabbit BCMA, His Tag (Cat. No. BCA-R52H4) at 2 μg/mL (100 μL/well) can bind Human BAFF, Fc Tag, active trimer (Cat. No. BAF-H5261) with a linear range of 0.4-6 ng/mL (Routinely tested).

Protocol
  • Background
    Tumor necrosis factor receptor superfamily member 17 (TNFRSF17) is also known as B-cell maturation protein (BCMA), CD antigen CD269, which is a member of the TNF-receptor superfamily. TNFRSF17 contains one TNFR-Cys repeat. TNFRSF17 is expressed in mature B-cells, but not in T-cells or monocytes. TNFRSF17 is receptor for TNFSF13B/BLyS/BAFF and TNFSF13/APRIL. TNFRSF17 promotes B-cell survival and plays a role in the regulation of humoral immunity. TNFRSF17 can activate NF-kappa-B and JNK.
  • Clinical and Translational Updates
      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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