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Your Position: Home > Cytokines > IL-7 > IL7-H5253

Human IL-7 Protein, Fc Tag (MALS verified)

  • Synonym
    IL7,Interleukin-7
  • Source
    Human IL-7, Fc Tag (IL7-H5253) is expressed from human 293 cells (HEK293). It contains AA Asp 26 - His 177 (Accession # P13232-1).
    Predicted N-terminus: Asp 26
  • Molecular Characterization
    Online(Asp 26 - His 177) P13232-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 43.8 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-7, Fc Tag (Cat. No. IL7-H5253) SDS-PAGE gel

Human IL-7, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human IL-7, Fc Tag (Cat. No. ) MALS images

The purity of Human IL-7, Fc Tag (Cat. No. IL7-H5253) was more than 85% and the molecular weight of this protein is around 107-120 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human IL-7, Fc TagHuman IL-7, Fc Tag (Cat. No. IL7-H5253) ELISA bioactivity

Immobilized Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. IL7-H5258) at 2 μg/mL (100 μL/well) can bind Human IL-7, Fc Tag (Cat. No. IL7-H5253) with a linear range of 0.3-5 ng/mL (QC tested).

  • Background
    Interleukin 7 is also known as IL7, IL-7, and is a hematopoietic growth factor secreted by stromal cells in the red marrow and thymus. It is also produced by keratinocytes, dendritic cells, hepatocytes, neurons, and epithelial cells, but is not produced by lymphocytes. IL-7 stimulates the differentiation of multipotent (pluripotent) hematopoietic stem cells into lymphoid progenitor cells, It also stimulates proliferation of all cells in the lymphoid lineage (B cells, T cells and NK cells). It is important for proliferation during certain stages of B-cell maturation, T and NK cell survival, development and homeostasis. IL-7 is a cytokine important for B and T cell development. This cytokine and the hepatocyte growth factor (HGF) form a heterodimer that functions as a pre-pro-B cell growth-stimulating factor. IL-7 binds to the IL-7 receptor, a heterodimer consisting of Interleukin-7 receptor alpha and common gamma chain receptor. Il-7 promotes hematological malignacies (acute lymphoblastic leukemia, T cell lymphoma). Elevated levels of IL-7 have also been detected in the plasma of HIV-infected patients. IL-7 as an immunotherapy agent has been examined in many pre-clinical animal studies and more recently in human clinical trials for various malignancies and during HIV infection. IL-7 could also be beneficial in improving immune recovery after allogenic stem cell transplant.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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