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Your Position: Home > Viral Proteins > Spike RBD > SPD-C5256

SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (MALS verified)

  • Synonym
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD
  • Source
    SARS-CoV-2 S protein RBD (K417N,E484K,N501Y), Fc Tag (SPD-C5256) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1(K417N, E484K, N501Y)). The K417N, E484K, N501Y mutations were identified in the SARS-CoV-2 Beta variant (Pango lineage: B.1.351; other names: 20H/501Y.V2).
    Predicted N-terminus: Arg 319
  • Molecular Characterization
    Online(Arg 319 -  Lys 537) QHD43416.1 (K417N, E484K, N501Y)

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 51.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) SDS-PAGE gel

SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. ) MALS images

The purity of SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) was more than 90% and the molecular weight of this protein is around 110-130 kDa verified by SEC-MALS.

Bioactivity-ELISA
SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc TagSARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) ELISA bioactivity

Immobilized SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) at 2 μg/mL (100 μL/well) can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with a linear range of 39-156 ng/mL (QC tested).

SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc TagSARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) ELISA bioactivity

Immobilized Human ACE2, His Tag (Cat. No. AC2-H52H8) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 S protein RBD (K417N, E484K, N501Y), Fc Tag (Cat. No. SPD-C5256) with a linear range of 0.1-0.8 ng/mL (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded SARS-CoV-2 S protein RBD, Fc Tag (Cat. No. SPD-C5256) on Protein A Biosensor, can bind Human ACE2, His Tag (Cat. No. AC2-H52H8) with an affinity constant of 2.03 nM as determined in BLI assay (ForteBio Octet Red96e)(Routinely tested).

  • Background
    It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $400.00

Price(USD) : $2600.00

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