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Your Position: Home > Protein > LIV-1 > LV1-H82E3

Biotinylated Human LIV-1 / SLC39A6 Protein, His,Avitag™

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  • Synonym
    SLC39A6,LIV-1,ZIP6,Zinc transporter ZIP6,ZIP-6
  • Source
    Biotinylated Human LIV-1, His,Avitag (LV1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Phe 29 - Trp 325 (Accession # Q13433-1).
    Predicted N-terminus: Phe 29
  • Molecular Characterization
    Online(Phe 29 - Trp 325) Q13433-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 37.2 kDa. The protein migrates as 55-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human LIV-1, His,Avitag (Cat. No. LV1-H82E3) SDS-PAGE gel

Biotinylated Human LIV-1, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human LIV-1, His,AvitagBiotinylated Human LIV-1, His,Avitag (Cat. No. LV1-H82E3) ELISA bioactivity

Immobilized Biotinylated Human LIV-1, His,Avitag (Cat. No. LV1-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-LIV-1 Antibody, Human IgG1 with a linear range of 0.6-1 ng/mL (QC tested).

  • Background
    LIV-1 is also known as SLC39A6, ZIP-6 and Zinc transporter ZIP6. May act as a zinc-influx transporter. Highly expressed in the breast, prostate, placenta, kidney, pituitary and corpus callosum. Weakly expressed in heart and intestine. Also highly expressed in cells derived from an adenocarcinoma of the cervix and lung carcinoma. Up-regulated by estrogen in breast cancer cells lines.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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  • Latest Research Phase:Phase 2 Clinical

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