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Product Details
Synonyms
CAIX, CA9, CA-IX, G250, MN, P54, 58N, pMW1
Source
Biotinylated Human Carbonic Anhydrase IX (38-414), His,Avitag (CA9-H82E3) is expressed from human 293 cells (HEK293). It contains AA Gln 38 - Asp 414 (Accession # Q16790-1).
Predicted N-terminus: Gln 38
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 44.5 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a dimer.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 20 mM MES, 100 mM NaCl, pH6.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Biotinylated Human Carbonic Anhydrase IX (38-414), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS
The purity of Biotinylated Human Carbonic Anhydrase IX (38-414), His,Avitag (Cat. No. CA9-H82E3) is more than 95% and the molecular weight of this protein is around 86-115 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated Human Carbonic Anhydrase IX (38-414), His,Avitag (Cat. No. CA9-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-Carbonic Anhydrase IX Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Anti-Carbonic Anhydrase IX Antibody captured on Protein A Chip can bind Biotinylated Human Carbonic Anhydrase IX (38-414), His,Avitag (Cat. No. CA9-H82E3) with an affinity constant of 2.97 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity
The specific activity is >40 pmol/min/μg, as measured with 4-Nitrophenyl acetate (Routinely tested). One unit is defined as the amount of enzyme that hydrolyze 1.0 p mole of 4-Nitrophenyl acetate to 4-Nitrophenol per minute at pH7.5 at room temperature.
Protocol
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Background
Carbonic anhydrase 9 (CA9 / CAIX) is also known as Membrane antigen MN (MN), Renal cell carcinoma-associated antigen G250, which belongs to the alpha-carbonic anhydrase family. CA9 / CAIX with an optimal activity at pH 6.49. Reversible hydration of carbon dioxide. CA IX participates in pH regulation. CA9 may be involved in the control of cell proliferation and transformation. CA-IX appears to be a novel specific biomarker for a cervical neoplasia.
Recent Advances
Drug Development Progress- English Name：
Carbonic anhydrase IX
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
16 Details
- Highest Development Stage：
Phase 3 Clinical
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