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Your Position: Home > Kits > Spike RBD > RAS-T014

Anti-SARS-CoV-2 Antibody IgG1 Titer Serologic Assay kit

For research use only.
Materials Provided
IDComponentsSize
RAS014-C01Pre-coated SARS-CoV-2 Spike RBD Microplate1 plate
RAS014-C02Anti-SARS-CoV-2 Antibody (Control, IgG1)100 μL
RAS014-C03HRP-Mouse anti-Human IgG120 μL
RAS014-C0410xWashing Buffer 50 mL
RAS014-C05Dilution Buffer50 mL
RAS014-C06Substrate Solution12 mL
RAS014-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) poses continual threat to human health due to rapid transmission worldwide. Unprecedented vaccination campaigns have now begun with multiple candidates. To support these studies, ACRO has developed Anti-SARS-CoV-2 subtype antibody Serologic Assay kit including:IgG1, IgG2 IgG3, IgG4.
  • Application
    This kit is developed for serologic test for IgG1 titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
  • Storage

    This kit is shipped at room temperature. The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.

  • Assay Principles
    This assay kit is used to measure the levels of Anti-SARS-CoV-2 antibody IgG1 by employing an indirect ELISA format. The microplate in the kit has been pre-coated with SARS-CoV-2 Spike RBD. First add the diluted samples to the plate and wash the wells after incubation. Afterwards add HRP-Mouse anti-Human IgG1 to the plate and wash the wells after incubation. Lastly the substrate is loaded into the wells and color develops in proportion to the amount of antibody. The reaction is stopped by the addition of a stop solution and the intensity of the color can be measured at 450 nm. The OD Value reflects the amount of antibody bound.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Mouse-Anti-Human IgG1 to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calc μLated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgG1 Titer Serologic Assay kitAnti-SARS-CoV-2 Antibody IgG1 Titer Serologic Assay kit (Cat. No. RAS-T014) ELISA bioactivity

Detection of Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG1 antibody with sensitivity of 4 ng/mL (QC tested).

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Price(USD) : $580.00

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