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Your Position: Home > All Other Proteins > Integrin alpha 10 beta 1 > IT1-M52W4

Mouse Integrin alpha 10 beta 1 (ITGA10&ITGB1) Heterodimer Protein, His Tag&Tag Free

  • Synonym
    Integrin alpha 10 beta 1,ITGA10&ITGB1
  • Source
    Mouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-M52W4) is expressed from human 293 cells (HEK293). It contains AA Phe 23 - Thr 1118 (ITGA10) & Gln 21 - Asp 728 (ITGB1) (Accession # E9PXZ3-1(ITGA10) & P09055-1(ITGB1)).
    Predicted N-terminus: Phe 23 (ITGA10) & Gln 21 (ITGB1)
  • Molecular Characterization
    Online(Phe 23 - Thr 1118 (ITGA10) & Gln 21 - Asp 728 (ITGB1)) E9PXZ3-1 (ITGA10) & P09055-1 (ITGB1)

    Mouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA10 and ITGB1, has a calculated MW of 126.6 kDa (ITGA10) and 83.5 kDa (ITGB1). Subunit ITGA10 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The reducing (R) protein migrates as 150-170 kDa (ITGA10) and 100-130 kDa (ITGB1) respectively due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Mouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-M52W4) SDS-PAGE gel

Mouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Mouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag FreeMouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-M52W4) ELISA bioactivity

Immobilized Human Collagen IV at 2 μg/mL (100 μL/well) can bind Mouse ITGA10&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-M52W4) with a linear range of 0.039-1.25 μg/mL (QC tested).

  • Background
    Human integrin alpha(10)I domain as a recombinant protein to reveal its ligand binding specificity. In general, alpha(10)I did recognize collagen types I-VI and laminin-1 in a Mg(2+)-dependent manner, whereas its binding to tenascin was only slightly better than to albumin. Alpha 10 beta 1 is a known collagen-binding I domain integrin, in addition to α1β1, α2β1 and α11β1. GROGER found in the N-terminal domain of collagens I and III, is only weakly recognised by α10β1, an important collagen receptor on chondrocytes, contrasting with the other collagen-binding integrins.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $410.00

Price(USD) : $2880.00

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