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Your Position: Home > 2019-nCoV proteins > Spike RBD > RAS-T019

Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD)

For research use only.
Materials Provided
IDComponentsSize
RAS019-C01Pre-coated SARS-CoV-2 Spike RBD Microplate1 plate
RAS019-C02Anti-SARS-CoV-2 Antibody (Control, Monkey IgG)100 μL
RAS019-C03Positive Control100 μL
RAS019-C04Negative Control100 μL
RAS019-C05HRP-Goat anti-Monkey IgG 100 μL
RAS019-C0610xWashing Buffer 50 mL
RAS019-C07Dilution Buffer50 mL
RAS019-C08Substrate Solution12 mL
RAS019-C09Stop Solution7 mL
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide. The SARS-CoV-2 pandemic presents an extraordinary challenge to the lives and health of the global population, resulting in a total of over 200 million diagnosed cases and 4.9 million death (as of October 2021). It also significantly affected the social, economic and environmental domains throughout the world and changed everyone’s life. ACROBiosystems, a leading life science company dedicated to aid the global COVID-19 response,developed different kits suitable for serum antibody detection in different species (Mouse, Monkeys, humans) to meet the needs of preclinical and clinical experiments.
  • Application
    This kit is developed for serologic test for Monkey IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Monkey serum by SARS-CoV-2 Spike RBD . The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Anti-SARS-CoV-2 Antibody (Control, Monkey IgG), an Positive Control, an Negative Control, an HRP-Anti-Goat anti monkey IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Goat anti monkey IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD)Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD) (Cat. No. RAS-T019) ELISA bioactivity

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Monkey IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Monkey IgG in 1:50 monkey serum. Detection was performed using HRP-Goat anti-Monkey IgG antibody with sensitivity of 98 ng/mL (QC tested).

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Price(USD) : $580.00

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