Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD)

Materials Provided

Application

This kit is developed for serologic test for Monkey IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.

It is for research use only.

Reconstitution

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

Storage

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Assay Principles

This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Monkey serum by SARS-CoV-2 Spike RBD . The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Anti-SARS-CoV-2 Antibody (Control, Monkey IgG), an Positive Control, an Negative Control, an HRP-Anti-Goat anti monkey IgG secondary antibody and related buffer.

Your experiment will include 4 simple steps:

a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

b) Add diluted Secondary antibody HRP-Anti-Goat anti monkey IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

ACRO Quality Management System

1. QMS(ISO, GMP)
2. Quality Advantages
3. Quality Control Process