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Your Position: Home > 2019-nCoV proteins > Spike RBD > RAS-T019

Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD)

For research use only.
Materials Provided
IDComponentsSize
RAS019-C01Pre-coated SARS-CoV-2 Spike RBD Microplate1 plate
RAS019-C02Anti-SARS-CoV-2 Antibody (Control, Monkey IgG)100 μL
RAS019-C03Positive Control100 μL
RAS019-C04Negative Control100 μL
RAS019-C05HRP-Goat anti-Monkey IgG 100 μL
RAS019-C07Dilution Buffer50 mL
RAS019-C08Substrate Solution12 mL
RAS019-C09Stop Solution7 mL
  • Background
    The ongoing SARS-COV-2 pandemics has sickened 3.7 million people around the world, claiming over 1,000,000 lives. To deal with this global public health crisis, unprecedented efforts are being made to study the virus, SARS-CoV-2. At present, there are 193 new crown vaccine projects registered with the World Health Organization are being developed. To support these studies, ACRO has developed Monkey Anti-SARS-CoV-2 antibody IgG Serologic Assay kit.
  • Application
    This kit is developed for serologic test for Monkey IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in Monkey serum by SARS-CoV-2 Spike RBD . The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Anti-SARS-CoV-2 Antibody (Control, Monkey IgG), an Positive Control, an Negative Control, an HRP-Anti-Goat anti monkey IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Goat anti monkey IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD)Monkey Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD) (Cat. No. RAS-T019) ELISA bioactivity

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Monkey IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Monkey IgG in 1:50 monkey serum. Detection was performed using HRP-Goat anti-Monkey IgG antibody with sensitivity of 98 ng/mL (QC tested).

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Price(USD) : $580.00

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