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Your Position: Home > Antibody isotype kits > Spike RBD > RAS-T015

Anti-SARS-CoV-2 Antibody IgG2 Titer Serologic Assay kit

For research use only.
IDComponentsSize
RAS015-C01Pre-coated with SARS-CoV-2 Spike RBD Microplate1 plate
RAS015-C02Anti-SARS-CoV-2 Antibody (Control, IgG2)10 μg
RAS015-C03HRP-Mouse anti-Human IgG275 μL
RAS015-C0410 x Washing Buffer 50 mL
RAS015-C05Dilution Buffer50 mL
RAS015-C06Substrate Solution12 mL
RAS015-C07Stop Solution7 mL
  • Background
    The ongoing SARS-COV-2 pandemics has sickened 3.7 million people around the world, claiming over 1,000,000 lives. To deal with this global public health crisis, unprecedented efforts are being made to study the virus, SARS-CoV-2. At present, there are 193 new crown vaccine projects registered with the World Health Organization are being developed. To support these studies, ACRO has developed Anti-SARS-CoV-2 subtype antibody Serologic Assay kit including:IgG1, IgG2 IgG3, IgG4.
  • Application
    This kit is developed for serologic test for IgG2 titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.

    Below is the Shipping Statement for Kit Products.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The Kit consists of Pre-coated with SARS-CoV-2 Spike RBD Microplate, an Anti-SARS-CoV-2 Antibody (Control, IgG2), an HRP-Anti-Human IgG2 secondary antibody.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Mouse-Anti-Human IgG2 to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data Please refer to DS document for The assay protocol.
 Spike RBD ELISA

Detection of Anti-SARS-CoV-2 Antibody, Human IgG2 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG2 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG2 antibody with sensitivity of 1000 ng/mL (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $580.00

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