|RAS015-C01||Pre-coated with SARS-CoV-2 Spike RBD Microplate||1 plate|
|RAS015-C02||Anti-SARS-CoV-2 Antibody (Control, IgG2)||10 μg|
|RAS015-C03||HRP-Mouse anti-Human IgG2||75 μL|
|RAS015-C04||10 x Washing Buffer||50 mL|
|RAS015-C05||Dilution Buffer||50 mL|
|RAS015-C06||Substrate Solution||12 mL|
|RAS015-C07||Stop Solution||7 mL|
After reconstitution, the stock solution should be kept at -70°C.
It is recommended not to freeze thaw more than 3 times.
Your experiment will include 4 simple steps:
a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Mouse-Anti-Human IgG2 to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Anti-SARS-CoV-2 Antibody, Human IgG2 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG2 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG2 antibody with sensitivity of 1000 ng/mL (QC tested).
ACROBiosystems has developed an exclusive immune checkpoint protein collection， The consistency and excellent performance of our proteins are confirmed by a variety of assays.
ACROBiosystems has developed a series of homogeneous CD3 proteins with good bioactivity. They are verified as 1:1 heterodimers by nonreductive electrophoresis and MALS as well.
ACROBiosystems has developed a series of products with different species and tags for a variety of hot targets. It is suitable for immune, antibody screening, SPR, cell activity detection and other experiments, and all SPR&BLI protocols are available for free.
We also supply difficult-to-express proteins such as BCMA, CD19, ROR1, and EGFRVIII.
This web search service is supported by Google Inc.