|RAS015-C01||Pre-coated with SARS-CoV-2 Spike RBD Microplate||1 plate|
|RAS015-C02||Anti-SARS-CoV-2 Antibody (Control, IgG2)||10 μg|
|RAS015-C03||HRP-Mouse anti-Human IgG2||75 μL|
|RAS015-C04||10 x Washing Buffer||50 mL|
|RAS015-C05||Dilution Buffer||50 mL|
|RAS015-C06||Substrate Solution||12 mL|
|RAS015-C07||Stop Solution||7 mL|
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
Your experiment will include 4 simple steps:
a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.
b) Add diluted Secondary antibody HRP-Mouse-Anti-Human IgG2 to the plate. The Secondary antibody is diluted by Dilution Buffer.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.
Detection of Anti-SARS-CoV-2 Antibody, Human IgG2 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG2 in 1:50 human serum. Detection was performed using HRP-Conjugated Anti-human IgG2 antibody with sensitivity of 1000 ng/mL (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $580.00
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