|RAS020-C01||Pre-coated with Anti-SARS-CoV-2 Spike Protein Antibody Microplate||1 plate|
|RAS020-C02||SARS-CoV-2 Spike Protein||10 μg|
|RAS020-C03||Biotin-Anti-SARS-CoV-2 Spike Protein Antibody||10 μg|
|RAS020-C05||10xWashing Buffer||50 mL|
|RAS020-C06||Dilution Buffer||50 mL|
|RAS020-C07||Substrate Solution||12 mL|
|RAS020-C08||Stop Solution||7 mL|
The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the SARS-CoV-2 Spike protein as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add a diluted Secondary antibody biotin-Anti-SARS-CoV-2 Spike protein Antibody to the plate. The Secondary antibody is diluted by Dilution Buffer.
d) Add a diluted Streptavidin-HRP to the plate.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike protein. The kit consists of pre-coated Anti-SARS-CoV-2 Spike Protein Antibody Microplate, SARS-CoV-2 Spike protein as Control, biotin-Anti-SARS-CoV-2 Spike protein antibody and HRP-Streptavidin. Detection is performed using HRP-Streptavidin with sensitivity of 200 pg/mL.
SARS-CoV-2 Spike Protein Titer Assay Kit (Cat.No.RAS-A020) can potently to detect the all SARS-CoV-2 Variants of Concern (VOCs), including Alpha (Cat.No.SPN-C52H6), Beta (Cat.No.SPN-C52Hk), Gamma (Cat.No.SPN-C52Hg) and Delta (Cat.No.SPN-C52He).
SARS-CoV-2 Spike Protein Titer Assay Kit (Cat.No.RAS-A020) can potently to detect the all SARS-CoV-2 Variants of Concern (VOCs), including Omicron (Cat.No.SPN-C52Hz).
Price(USD) : $580.00
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