| ID | Components | Size |
| RAS020-C01 | Pre-coated with Anti-SARS-CoV-2 Spike Protein Antibody Microplate | 1 plate |
| RAS020-C02 | SARS-CoV-2 Spike Protein | 10 μg |
| RAS020-C03 | Biotin-Anti-SARS-CoV-2 Spike Protein Antibody | 10 μg |
| RAS020-C04 | Streptavidin-HRP | 10 μg |
| RAS020-C05 | 10xWashing Buffer | 50 mL |
| RAS020-C06 | Dilution Buffer | 50 mL |
| RAS020-C07 | Substrate Solution | 12 mL |
| RAS020-C08 | Stop Solution | 7 mL |
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.
Below is the Shipping Statement for Kit Products.
Your experiment will include 6 simple steps:
a) All reagents were returned to room temperature(20°C-25°C) before use.
b) Add your sample to the plate, take the SARS-CoV-2 Spike protein as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add a diluted Secondary antibody biotin-Anti-SARS-CoV-2 Spike protein Antibody to the plate. The Secondary antibody is diluted by Dilution Buffer.
d) Add a diluted Streptavidin-HRP to the plate.
e) Wash the plate and add TMB or other colorimetric HRP substrate.
f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of SARS-CoV-2 Spike protein. The kit consists of pre-coated Anti-SARS-CoV-2 Spike Protein Antibody Microplate, SARS-CoV-2 Spike protein as Control, biotin-Anti-SARS-CoV-2 Spike protein antibody and HRP-Streptavidin. Detection is performed using HRP-Streptavidin with sensitivity of 200 pg/mL.
SARS-CoV-2 Spike Protein Titer Assay Kit (Cat.No.RAS-A020) can potently to detect the all SARS-CoV-2 Variants of Concern (VOCs), including Alpha (Cat.No.SPN-C52H6), Beta (Cat.No.SPN-C52Hk), Gamma (Cat.No.SPN-C52Hg) and Delta (Cat.No.SPN-C52He).
SARS-CoV-2 Spike Protein Titer Assay Kit (Cat.No.RAS-A020) can potently to detect the all SARS-CoV-2 Variants of Concern (VOCs), including Omicron (Cat.No.SPN-C52Hz).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
Price(USD) : $580.00
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