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Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 18.5 kDa (IL-17A) and 16.8 kDa (IL-17F). Subunit IL-17A is fused with a Twin Strep tag at the N-terminus and subunit IL-17F is fused with a polyhistidine tag at the N-terminus. The reducing (R) protein migrates as 19-27 kDa due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) is more than 85% and the molecular weight of this protein is around 35-52 kDa verified by SEC-MALS.
Immobilized Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (MALS verified) (Cat. No. ILA-H5257) with a linear range of 0.6-10 ng/mL (QC tested).
Immobilized Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 5-78 ng/mL (Routinely tested).
Immobilized Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL17A Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).
Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) captured on CM5 chip via anti-human IgG Fc antibody can bind Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) with an affinity constant of 50.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) with an affinity constant of 3.70 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 92.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) stimulates production of IL-6 in NIH-3T3 cells in the presence of 200 ng/mL human TNF-alpha. The EC50 for this effect is 11.34-12.46 ng/mL (Routinely tested).
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