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Your Position: Home > Cytokines > IL-17A & IL-17F > ILF-H52W6

Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (MALS verified)

  • Synonym
    IL-17A,Interleukin-17A,CTLA-8,IL-17,IL-17F,Interleukin-17F,Cytokine ML-1
  • Source
    Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (ILF-H52W6) is expressed from human 293 cells (HEK293). It contains AA Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F) (Accession # Q16552-1(IL-17A) & Q96PD4-1(IL-17F)). Two sequential affinity purification steps were used to ensure exact 1:1 molar ratio of IL17A&IL17F heterodimer.
    Predicted N-terminus: Trp (IL-17A) & His (IL-17F)
  • Molecular Characterization
    Online(Gly 24 - Ala 155 (IL-17A) & Arg 31 - Gln 163 (IL-17F)) Q16552-1 (IL-17A) & Q96PD4-1 (IL-17F)

    Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag, produced by co-expression of IL-17A and IL-17F, has a calculated MW of 18.5 kDa (IL-17A) and 16.8 kDa (IL-17F). Subunit IL-17A is fused with a Twin Strep tag at the N-terminus and subunit IL-17F is fused with a polyhistidine tag at the N-terminus. The reducing (R) protein migrates as 19-27 kDa due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) SDS-PAGE gel

Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ) MALS images

The purity of Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) was more than 85% and the molecular weight of this protein is around 42-52 kDa verified by SEC-MALS.

Bioactivity-ELISA
Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His TagHuman IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) ELISA bioactivity

Immobilized Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) at 2 μg/mL (100 μL/well) can bind Human IL-17 RA, Fc Tag (MALS verified) (Cat. No. ILA-H5257) with a linear range of 0.6-10 ng/mL (QC tested).

Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His TagHuman IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) ELISA bioactivity

Immobilized Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) at 2 μg/mL (100 μL/well) can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with a linear range of 5-78 ng/mL (Routinely tested).

Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His TagHuman IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) ELISA bioactivity

Immobilized Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human IL17A Antibody, Human IgG1 with a linear range of 0.1-4 ng/mL (Routinely tested).

Bioactivity-SPR
Human_FcRn_Heterodimer_Protein_SPR

Human IL17RA & IL17RC Protein, Fc Tag&Fc Tag (Cat. No. ILC-H5257) captured on CM5 chip via anti-human IgG Fc antibody can bind Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) with an affinity constant of 50.8 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Human IL-17 RA, Fc Tag (Cat. No. ILA-H5257) on Protein A Biosensor, can bind Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) with an affinity constant of 3.70 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Human_FcRn_Heterodimer_Protein_Bli

Loaded Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) on NTA Biosensor, can bind Human IL-17 RC, Fc Tag (Cat. No. ILC-H5259) with an affinity constant of 92.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Bioactivity-Cell based assay
Human FcRn Heterodimer Protein Cell_Base

Human IL-17A&IL-17F Heterodimer Protein, Twin Strep&His Tag (Cat. No. ILF-H52W6) stimulates production of IL-6 in NIH-3T3 cells in the presence of 200 ng/mL human TNF-alpha. The EC50 for this effect is 11.34-12.46 ng/mL (Routinely tested).

  • Background
    Interleukin-17A (IL17A) is also known as cytotoxic T-lymphocyte-associated antigen 8 (CTLA8),which is a proinflammatory cytokine produced by activated T cells. IL17A can regulate the activities of NF-kappaB and mitogen-activated protein kinases. Also,IL17A can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO).Furthermore,IL17A has been found both in glycosylated and nonglycosylated forms. High levels of IL-17 are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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