Save Product
Product Details
Synonyms
FGF2, BFGF, FGFB, FGF basic, HBGF-2
Source
Biotinylated Human FGF basic, Avitag,His Tag (FGC-H81E3) is expressed from E. coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag. The protein has a calculated MW of 20.1 kDa. The protein migrates as 20-22 kDa under reducing (R) condition (SDS-PAGE).
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Biotinylated Human FGF basic, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-HPLC
The purity of Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) was greater than 85% as determined by SEC-HPLC.
Bioactivity-ELISA
Immobilized Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.6-10 ng/mL (QC tested).
Protocol
Immobilized Human FGFR2 (IIIb), His Tag (Cat. No. FGB-H5223) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Protocol
Immobilized Human FGFR2 (IIIc), His Tag (Cat. No. FGC-H5225) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 5-39 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) immobilized on SA Chip can bind Human FGF R1, His Tag (Cat. No. FG1-H5223) with an affinity constant of 96.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Fibroblast growth factor 2
- Category：
- Approved Drugs：
1 Details
- Drugs in Clinical Trials：
4 Details
- Highest Development Stage：
Approved
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
