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Your Position: Home > Cytokines > FGF basic > FGC-H81E3

Biotinylated Human FGF basic Protein, Avitag™,His Tag (MALS verified)

  • Synonym
    FGF2,BFGF,FGFB,FGF basic,HBGF-2
  • Source
    Biotinylated Human FGF basic, Avitag,His Tag (FGC-H81E3) is expressed from E.coli cells. It contains AA Pro 143 - Ser 288 (Accession # P09038-4).
    Predicted N-terminus: Met
  • Molecular Characterization
    Online(Pro 143 - Ser 288) P09038-4

    This protein carries an Avi tag at the N-terminus, followed by a polyhistidine tag.

    The protein has a calculated MW of 20.1 kDa. The protein migrates as 20-22 kDa under reducing (R) condition (SDS-PAGE).

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl,pH7.5 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) SDS-PAGE gel

Biotinylated Human FGF basic, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. ) MALS images

The purity of Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) was more than 85% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human FGF basic, Avitag,His TagBiotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) ELISA bioactivity

Immobilized Human Glypican 3, Fc Tag, low endotoxin (Cat. No. GP3-H5258) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.6-10 ng/mL (QC tested).

Biotinylated Human FGF basic, Avitag,His TagBiotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) ELISA bioactivity

Immobilized Human FGFR2 (IIIb), His Tag (Cat. No. FGB-H5223) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).

Biotinylated Human FGF basic, Avitag,His TagBiotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) ELISA bioactivity

Immobilized Human FGFR2 (IIIc), His Tag (Cat. No. FGC-H5225) at 5 μg/mL (100 μL/well) can bind Biotinylated Human FGF basic, Avitag,His Tag (Cat. No. FGC-H81E3) with a linear range of 5-39 ng/mL (Routinely tested).

  • Background
    FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60% amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway. FGF basic has been isolated from a number of sources, including neural tissue, pituitary, adrenal cortex, corpus luteum, and placenta. This factor contains four cysteine residues, but reduced FGF basic retains full biological activity, indicating that disulfide bonds are not required for this activity. bFGF is a critical component of human embryonic stem cell culture medium; the growth factor is necessary for the cells to remain in an undifferentiated state, although the mechanisms by which it does this are poorly defined. It has been demonstrated to induce gremlin expression which in turn is known to inhibit the induction of differentiation by bone morphogenetic proteins. It is necessary in mouse-feeder cell dependent culture systems, as well as in feeder and serum-free culture systems.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $250.00

Price(USD) : $980.00

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  • Number of Launched Drugs:3 Details
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  • Latest Research Phase:Approved

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