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Your Position: Home > All Other Proteins > Gastrin-17 > GA7-H82F4

Biotinylated Human Gastrin-17 Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    gastrin-17,gastrin 17,gastrin
  • Source
    Biotinylated Human Gastrin-17, Fc,Avitag (GA7-H82F4) is expressed from human 293 cells (HEK293).
  • Molecular Characterization

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 30.3 kDa. The protein migrates as 34-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Gastrin-17, Fc,Avitag (Cat. No. GA7-H82F4) SDS-PAGE gel

Biotinylated Human Gastrin-17, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human Gastrin-17, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human Gastrin-17, Fc,Avitag (Cat. No. GA7-H82F4) was more than 90% and the molecular weight of this protein is around 60-70 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human Gastrin-17, Fc,AvitagBiotinylated Human Gastrin-17, Fc,Avitag (Cat. No. GA7-H82F4) ELISA bioactivity

Immobilized Anti-Gastrin-17 antibody, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human Gastrin-17, Fc,Avitag (Cat. No. GA7-H82F4) with a linear range of 0.1-2.5 ng/mL (QC tested).

  • Background
    Gastrin is a peptide hormone that stimulates secretion of gastric acid (HCl) by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the pyloric antrum of the stomach, duodenum, and the pancreas. Gastrin binds to cholecystokinin B receptors to stimulate the release of histamines in enterochromaffin-like cells, and it induces the insertion of K+/H+ ATPase pumps into the apical membrane of parietal cells (which in turn increases H+ release into the stomach cavity). Its release is stimulated by peptides in the lumen of the stomach.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 3 Clinical

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