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Your Position: Home > All Other Proteins > CD72 > CD2-H82E3

Biotinylated Human CD72 Protein, His,Avitag™ (MALS verified)

Based on the high binding affinity between biotin-streptavidin systems, our best seller SP-11 Streptavidin Coated Plates are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
  • Synonym
    CD72,Lyb-2,Lyb2
  • Source
    Biotinylated Human CD72, His,Avitag (CD2-H82E3) is expressed from human 293 cells (HEK293). It contains AA Arg 117 - Asp 359 (Accession # P21854-1).
    Predicted N-terminus: Arg 117
  • Molecular Characterization
    Online(Arg 117 - Asp 359) P21854-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 31.7 kDa. The protein migrates as 34-38 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human CD72, His,Avitag (Cat. No. CD2-H82E3) SDS-PAGE gel

Biotinylated Human CD72, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human CD72, His,Avitag (Cat. No. ) MALS images

The purity of Human Siglec-3, His Tag (Cat. No. CD3-H5226) is more than 95% and the molecular weight of this protein is around 75-100 kDa verified by SEC-MALS.

  • Background
    B-cell differentiation antigen CD72 is also known as Lyb-2. CD72 contains one C-type lectin domain. CD72 is a protein active in the immune system of animals. CD72 consists of two identical halves, each of about 39-43 kD, and is a C-type lectin. Its primarily locus of expression is B-cells, where it appears to mediate aspects of B-cell - T-cell interaction. CD72 plays a role in B-cell proliferation and differentiation. CD72 is a ligand for CD5.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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