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Your Position: Home > CAR-T > CD7 > CD7-HP2E3

PE-Labeled Human CD7 Protein, His Tag (Site-specific conjugation)

  • Synonym
    CD7,GP40,TP41,LEU-9,Tp40
  • Source
    PE-Labeled Human CD7, His Tag (CD7-HP2E3) is produced via site-specific conjugation of PE to Human CD7 (26-180), His Tag under optimal conditions with a proprietary technology. Human CD7, His Tag (CD7-HP2E3) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Pro 180 (Accession # P09564-1).
    Predicted N-terminus: Ala 26
  • Molecular Characterization
    Online(Ala 26 - Pro 180) P09564-1

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 20.0 kDa.

  • Application
    Please note that this product is NOT compatible to streptavidin detection system.
  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-FACS
FACS assay shows that recombinant Biotinylated FACS

5e5 of anti-CD7 CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human CD7 Protein, His Tag(Cat. No. CD7-HP2E3) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

  • Background
    T-cell antigen CD7 (CD7) is also known as GP40, LEU-9, TP41 and Tp40. CD7 is a protein that in humans is encoded by the CD7 gene, this gene encodes a transmembrane protein which is a member of the immunoglobulin superfamily. CD7 has been shown to interact with PIK3R1. This protein is found on thymocytes and mature T cells. It plays an essential role in T-cell interactions and also in T-cell/B-cell interaction during early lymphoid development.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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