Biotinylated Human DPPIV / CD26 Protein, His,Avitag™ (active enzyme, MALS verified)

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Cat. No. / Size
Price
Qty
DP4-H82E3-25ug
$360.00
DP4-H82E3-200ug
$1565.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    DPP4, ADABP, ADCP2, CD26, DPPIV, TP103

  • Source

    Biotinylated Human DPPIV, His,Avitag (DP4-H82E3) is expressed from human 293 cells (HEK293). It contains AA Asp 34 - Pro 766 (Accession # NP_001926.2).

    Predicted N-terminus: Asp 34

    Request for sequence
  • Molecular Characterization

    DPPIV Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 88.4 kDa. The protein migrates as 100-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    DPPIV SDS-PAGE

    Biotinylated Human DPPIV, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    DPPIV SEC-MALS

    The purity of Biotinylated Human DPPIV, His,Avitag (Cat. No. DP4-H82E3) is more than 85% and the molecular weight of this protein is around 200-230 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     DPPIV ELISA

    Immobilized MERS S1 protein, His Tag (Cat. No. S1N-M52H5) at 2 μg/mL (100 μL/well) can bind Biotinylated Human DPPIV, His,Avitag (Cat. No. DP4-H82E3) with a linear range of 0.8-25 ng/mL (Routinely tested).

    Protocol
  • Bioactivity

    Measured by its ability to cleave the fluorogenic peptide substrate, Gly-Pro-7-amido-4-methylcoumarin (GP-AMC). The specific activity is >7500 pmol/min/µg (QC tested).

    Protocol

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Background

Dipeptidyl peptidase-IV (DPPIV) is also known as adenosine deaminase complexing protein 2, DPPIV or CD26 is antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. The substrates of DPPIV are proline (or alanine)-containing peptides and include growth factors, chemokines, neuropeptides, and vasoactive peptides. DPPIV plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1. DPPIV plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum increased in some neoplasms and decreased in others. DPPIV also binds the enzyme adenosine deaminase specifically and with high affinity. The significance of this interaction has yet to be established.

Recent Advances

 
Drug Development Progress
  • English Name:

    Dipeptidyl peptidase IV

  • Category:

  • Approved Drugs:

    41 Details

  • Drugs in Clinical Trials:

    40 Details

  • Highest Development Stage:

    Approved

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