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Your Position: Home > Cytokines > IL-11 > IL1-H82E3

Biotinylated Human IL-11 Protein, His,Avitag™ (MALS verified)

  • Synonym
    IL-11,Interleukin-11,AGIF,Oprelvekin,IL11
  • Source
    Biotinylated Human IL-11, His,Avitag (IL1-H82E3) is expressed from human 293 cells (HEK293). It contains AA Pro 22 - Leu 199 (Accession # P20809-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Pro 22 - Leu 199) P20809-1

    This protein carries a polyhistidine tag at the N-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 22.7 kDa. The protein migrates as 25-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5 M Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human IL-11, His,Avitag (Cat. No. IL1-H82E3) SDS-PAGE gel

Biotinylated Human IL-11, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Biotinylated Human IL-11, His,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Human IL-11, His,Avitag (Cat. No. IL1-H82E3) was more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human IL-11, His,AvitagBiotinylated Human IL-11, His,Avitag (Cat. No. IL1-H82E3) ELISA bioactivity

Immobilized Biotinylated Human IL-11, His,Avitag (Cat. No. IL1-H82E3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5. μg/well) plate can bind Human IL-11 R alpha, Fc Tag (Cat. No. ILR-H5256) with a linear range of 0.4-13 ng/mL (QC tested).

  • Background
    Interleukin-11 (IL-11) is a pleiotropic cytokine that stimulates megakaryocytopoiesis, resulting in increased production of platelets, as well as activating osteoclasts, inhibiting epithelial cell proliferation and apoptosis, and inhibiting macrophage mediator production. These functions may be particularly important in mediating the hematopoietic, osseous and mucosal protective effects of IL-11. The cytokine also possesses anti-inflammatory activity, and has been proposed as a therapeutic agent in the treatment of chronic inflammatory diseases, such as Crohn's disease and rheumatoid arthritis. Although IL-11 was initially believed to be restricted to mammals, subsequent studies demonstrated it to be expressed in fish. Despite close similarity in gene structure and conservation of key amino acids between fish and mammalian IL-11, they share relatively low overall amino acid identity and may not necessarily be functionally analogous.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $1380.00

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