Human GPA33 / A33 Protein, Fc Tag

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Cat. No. / Size
Price
Qty
GP3-H5253-50ug
$270.00
GP3-H5253-1mg
$1925.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    GPA33, A33

  • Source

    Human GPA33, Fc Tag (GP3-H5253) is expressed from human 293 cells (HEK293). It contains AA Ile 22 - Val 235 (Accession # Q99795-1).

    Predicted N-terminus: Ile 22

    Request for sequence
  • Molecular Characterization

    GPA33 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 50.1 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    GPA33 SDS-PAGE

    Human GPA33, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     GPA33 ELISA

    Immobilized Human GPA33, Fc Tag (Cat. No. GP3-H5253) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human GPA33 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).

    Protocol

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Background

The glycoprotein encoded by this gene is a cell surface antigen that is expressed in greater than 95% of human colon cancers. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 2 domains characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily.

Recent Advances

 
Drug Development Progress
  • English Name:

    Cell surface A33 antigen

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    0 Details

  • Highest Development Stage:

    Preclinical

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