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Your Position: Home > Immune Checkpoint Proteins > Nectin-4 > NE4-R5255

Rat Nectin-4 Protein, Fc Tag (MALS verified)

  • Synonym
    NECTIN4,LNIR, PRR4, PVRL4
  • Source
    Rat Nectin-4, Fc Tag (NE4-R5255) is expressed from human 293 cells (HEK293). It contains AA Tyr 28 - Val 348 (Accession # D3ZET1-1).
    Predicted N-terminus: Tyr 28
  • Molecular Characterization
    Online(Tyr 28 - Val 348) D3ZET1-1

    This protein carries a human IgG1 Fc tag at the C-terminus.

    The protein has a calculated MW of 61.1 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat Nectin-4, Fc Tag (Cat. No. NE4-R5255) SDS-PAGE gel

Rat Nectin-4, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Rat Nectin-4, Fc Tag (Cat. No. ) MALS images

The purity of Rat Nectin-4, Fc Tag (Cat. No. NE4-R5255) was more than 90% and the molecular weight of this protein is around 125-140 kDa verified by SEC-MALS.

Bioactivity-ELISA
Rat Nectin-4, Fc TagRat Nectin-4, Fc Tag (Cat. No. NE4-R5255) ELISA bioactivity

Immobilized Rat Nectin-4, Fc Tag (Cat. No. NE4-R5255) at 1 μg/mL (100 μL/well) can bind Anti-Nectin-4 Antibody, Mouse IgG1 with a linear range of 0.039-0.625 μg/mL (QC tested).

  • Background
    Nectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Order Details

Price(USD) : $350.00

Price(USD) : $2310.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

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