Biotinylated Human ITGA7X2B & ITGB1 Heterodimer Protein, produced by co-expression of ITGA7X2B and ITGB1, has a calculated MW of 116.9 kDa (ITGA7X2B) and 86.7 kDa (ITGB1). Subunit ITGA7X2B is fused with an acidic tail but no tag at the C-terminus and subunit ITGB1 contains a basic tail at the C-terminus and followed by a polyhistidine tag and an Avi tag (Avitag™). The non-reducing (NR) protein migrates as 100-115 kDa due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human ITGA7X2B&ITGB1 Heterodimer Protein, Tag Free&His,Avitag on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
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