Human Latent GDF-2 / BMP-9 Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
GD2-H52H3-50ug
$595.00
GD2-H52H3-1mg
$3400.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    Latent GDF-2, Latent GDF2, Latent BMP-9, Latent BMP9

  • Source

    Human Latent GDF-2, His Tag (GD2-H52H3) is expressed from human 293 cells (HEK293). It contains AA Lys 23 - Arg 429 (Accession # Q9UK05-1).

    Predicted N-terminus: His

    Request for sequence
  • Molecular Characterization

    Latent GDF-2 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 47.0 kDa. The protein migrates as 13 kDa and 40-45 kDa under reducing (R) condition (SDS-PAGE).

    The protein is designed as a dimer.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    After reconstitution, this product is stable after storage at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    Latent GDF-2 SDS-PAGE

    Human Latent GDF-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    Latent GDF-2 SEC-MALS

    The purity of Human Latent GDF-2, His Tag (Cat. No. GD2-H52H3) is more than 95% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.

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Background

Human Growth and differentiation factor 2 (GDF-2), also known as Bone morphogenetic protein 9 (BMP-9), is a member of the BMP subgroup of the TGF-beta superfamily proteins that signal through heterodimeric complexes composed of type I and type II BMP receptors. GDF-2 Potent circulating inhibitor of angiogenesis. Signals through the type I activin receptor ACVRL1 but not other Alks. Signaling through SMAD1 in endothelial cells requires TGF-beta coreceptor endoglin/ENG. ALK1 is a signalling receptor for bone morphogenetic protein-9 (BMP-9) in endothelial cells (ECs). BMP-9 bound with high affinity to ALK1 and endoglin, and weakly to the type-I receptor ALK2 and to the BMP type-II receptor (BMPR-II) and activin type-II receptor (ActR-II) in transfected COS cells. Binding of BMP-9 to ALK2 was greatly facilitated when BMPR-II or ActR-II were co-expressed.

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