Based on the high binding affinity between biotin-streptavidin systems, our Streptavidin series products are recommended to use together with biotinylated proteins to get lower non-specific binding results in a shorter time.
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™)
The protein has a calculated MW of 28.3 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) is more than 85% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.
Immobilized Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-1 ng/mL (QC tested).
Immobilized Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.4-1 ng/mL (Routinely tested).
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) with a linear range of 20-78 ng/mL (Routinely tested).
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) with a linear range of 0.2-1 ng/mL (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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