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Product Details
Synonyms
Spike, S protein RBD, Spike glycoprotein Receptor-binding domain, S glycoprotein RBD, Spike protein RBD
Source
Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (SPD-C82Ed) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (L452R, T478K)). The mutations L452R, T478K were identified in the SARS-CoV-2 Delta variant (Pango lineage: B.1.617.2; other names: 21A/S:478K).
Predicted N-terminus: Arg 319
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 28.3 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
SEC-MALS
The purity of Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) is more than 85% and the molecular weight of this protein is around 35-45 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human ACE2, Fc Tag (Cat. No. AC2-H5257) with a linear range of 0.4-1 ng/mL (QC tested).
Protocol
Immobilized Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) with a linear range of 0.4-1 ng/mL (Routinely tested).
Protocol
Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) with a linear range of 20-78 ng/mL (Routinely tested).
Protocol
Immobilized Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (Cat. No. S1N-M122) at 1 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 Spike RBD Protein (L452R, T478K), His,Avitag (Cat. No. SPD-C82Ed) with a linear range of 0.2-1 ng/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
G***e2022-05-27
LikesWe placed an order for Omicron RBD, to run side-by-side analyses of our neutralizing antibodies against Omicron vs Wild type/Wuhan RBD protein (also from Acro) in biolayer interferometry experiments. AcroBio's Biotinylated Omicron RBD protein loads onto streptavidin biosensors very well - achieving a 1nm spectral shift within 40 seconds using a 100nM solution. We were able to obtain satisfactory results that were consistent with findings from other orthogonal methods for characterizing our antibodies (ELISA, viral infectivity, etc). Protein arrived in a timely manner.
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