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Your Position: Home > 2019-nCoV proteins > Spike protein > RAS-T023

Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

For research use only.
Materials Provided
IDComponentsSize
RAS023-C01Pre-coated SARS-CoV-2 Spike Protein Microplate1 plate
RAS023-C02Anti-SARS-CoV-2 Antibody (Control, Mouse IgG )100 μL
RAS023-C03HRP-Goat anti-Mouse IgG10 μg
RAS023-C0410 x Washing Buffer50 mL
RAS023-C05Dilution Buffer50 mL
RAS023-C06Substrate Solution12 mL
RAS023-C07Stop Solution7 mL
RAS023-C08Positive Control100 μL
RAS023-C09Negative Control100 μL
  • Background
    SARS-CoV-2 is continuously circulating and posting challenges to human health. In response to this global pandemic, the Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Protein) is developed to help assess the antibody level induced by vaccines or viral challenge in mice model.
  • Application
    This kit is developed for detection of Mouse Anti-SARS-CoV-2 antibody (IgG) in serum in vitro.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt.  The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.

    It is recommended not to freeze thaw more than 3 times.

  • Assay Principles
    This kit is based on a standard indirect ELISA assay that allows rapid detection of Anti-SARS-CoV-2 antibodies (IgG) in mouse serum by SARS-CoV-2 Spike Protein. The kit consists of a Microplate Pre-coated with SARS-CoV-2 Spike Protein, Anti-SARS-CoV-2 Antibody (Control, Mouse IgG), and HRP-Goat-Anti-Mouse IgG Secondary Antibody.

    Your experiment will include 5 simple steps:

    a) Add your diluted Samples and Control to the plate.

    b) Add diluted Secondary Antibody HRP-Goat-Anti-Mouse IgG to the plate.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by adding diluted acid.

    e) To remove background noise prior to statistical analysis, absorbance (OD) is calculated by substracting the value at 450 nm with the value at 650 nm. The OD Value reflects the amount of antibody bound.

Typical Data
Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer) (Cat. No. RAS-T023) ELISA bioactivity

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG in 1:100 mouse serum. Detection was performed using HRP-Anti-mouse IgG antibody with sensitivity of 0.2 ng/mL (QC tested).

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Price(USD) : $580.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:2 Details
  • Latest Research Phase:Approved

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