|RAS023-C01||Pre-coated SARS-CoV-2 Spike Protein Microplate||1 plate|
|RAS023-C02||Anti-SARS-CoV-2 Antibody (Control, Mouse IgG )||100 μL|
|RAS023-C03||HRP-Goat anti-Mouse IgG||10 μg|
|RAS023-C04||10 x Washing Buffer||50 mL|
|RAS023-C05||Dilution Buffer||50 mL|
|RAS023-C06||Substrate Solution||12 mL|
|RAS023-C07||Stop Solution||7 mL|
|RAS023-C08||Positive Control||100 μL|
|RAS023-C09||Negative Control||100 μL|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 5 simple steps:
a) Add your diluted Samples and Control to the plate.
b) Add diluted Secondary Antibody HRP-Goat-Anti-Mouse IgG to the plate.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by adding diluted acid.
e) To remove background noise prior to statistical analysis, absorbance (OD) is calculated by substracting the value at 450 nm with the value at 650 nm. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG in 1:100 mouse serum. Detection was performed using HRP-Anti-mouse IgG antibody with sensitivity of 20 ng/mL (QC tested).
Emerging VoCs, Lambda, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
ELISA kits for neutralizing antibody titer assay, binding antibody titer assay, antibody isotype assay, antigen titer assay and inhibitor screening, etc., which can accelerate the research and development of anti-SARS-CoV-2 drugs and vaccines.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
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