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Your Position: Home > IgG kits > Spike protein > RAS-T023

Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

For research use only.
Materials Provided
IDComponentsSize
RAS023-C01Pre-coated SARS-CoV-2 Spike Protein Microplate1 plate
RAS023-C02Anti-SARS-CoV-2 Antibody (Control, Mouse IgG )100 μL
RAS023-C03HRP-Goat anti-Mouse IgG 10 μg
RAS023-C0410 x Washing Buffer 50 mL
RAS023-C05Dilution Buffer50 mL
RAS023-C06Substrate Solution12 mL
RAS023-C07Stop Solution7 mL
RAS023-C08Positive Control100 μL
RAS023-C09Negative Control100 μL
  • Background
    Since December 2019, the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and its associated disease, COVID-19, has caused a devastating pandemic worldwide. The SARS-CoV-2 pandemic presents an extraordinary challenge to the lives and health of the global population, resulting in a total of over 200 million diagnosed cases and 4.9 million death (as of October 2021). It also significantly affected the social, economic and environmental domains throughout the world and changed everyone’s life. ACROBiosystems, a leading life science company dedicated to aid the global COVID-19 response,developed different kits suitable for serum antibody detection in different species (Mouse, Monkeys, humans) to meet the needs of preclinical and clinical experiments.
  • Application
    This kit is developed for detection of Mouse Anti-SARS-CoV-2 antibody (IgG) in serum in vitro.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

  • Assay Principles
    This kit is based on a standard indirect ELISA assay that allows rapid detection of Anti-SARS-CoV-2 antibodies (IgG) in mouse serum by SARS-CoV-2 Spike Protein. The kit consists of a Microplate Pre-coated with SARS-CoV-2 Spike Protein, Anti-SARS-CoV-2 Antibody (Control, Mouse IgG), and HRP-Goat-Anti-Mouse IgG Secondary Antibody.

    Your experiment will include 5 simple steps:

    a) Add your diluted Samples and Control to the plate.

    b) Add diluted Secondary Antibody HRP-Goat-Anti-Mouse IgG to the plate.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by adding diluted acid.

    e) To remove background noise prior to statistical analysis, absorbance (OD) is calculated by substracting the value at 450 nm with the value at 630 nm. The OD Value reflects the amount of antibody bound.

Typical Data
Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer) (Cat. No. RAS-T023) ELISA bioactivity

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG in 1:100 mouse serum. Detection was performed using HRP-Anti-mouse IgG antibody with sensitivity of 20 ng/mL (QC tested).

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ETD of in-stock products: 2 business days

Price(USD) : $580.00

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:3 Details
  • Latest Research Phase:Approved

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