Mouse Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

This kit is developed for detection of Mouse Anti-SARS-CoV-2 antibody (IgG) in serum in vitro.

It is for research use only.

Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.

1. Unopened kit should be stored at 2℃-8℃ upon receiving.

2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

This kit is based on a standard indirect ELISA assay that allows rapid detection of Anti-SARS-CoV-2 antibodies (IgG) in mouse serum by SARS-CoV-2 Spike Protein. The kit consists of a Microplate Pre-coated with SARS-CoV-2 Spike Protein, Anti-SARS-CoV-2 Antibody (Control, Mouse IgG), and HRP-Goat-Anti-Mouse IgG Secondary Antibody.

Your experiment will include 5 simple steps:

a) Add your diluted Samples and Control to the plate.

b) Add diluted Secondary Antibody HRP-Goat-Anti-Mouse IgG to the plate.

c) Wash the plate and add TMB or other colorimetric HRP substrate.

d) Stop the substrate reaction by adding diluted acid.

e) To remove background noise prior to statistical analysis, absorbance (OD) is calculated by substracting the value at 450 nm with the value at 630 nm. The OD Value reflects the amount of antibody bound.