|RAS023-C01||Pre-coated SARS-CoV-2 Spike Protein Microplate||1 plate|
|RAS023-C02||Anti-SARS-CoV-2 Antibody (Control, Mouse IgG )||100 μL|
|RAS023-C03||HRP-Goat anti-Mouse IgG||10 μg|
|RAS023-C04||10 x Washing Buffer||50 mL|
|RAS023-C05||Dilution Buffer||50 mL|
|RAS023-C06||Substrate Solution||12 mL|
|RAS023-C07||Stop Solution||7 mL|
|RAS023-C08||Positive Control||100 μL|
|RAS023-C09||Negative Control||100 μL|
The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Your experiment will include 5 simple steps:
a) Add your diluted Samples and Control to the plate.
b) Add diluted Secondary Antibody HRP-Goat-Anti-Mouse IgG to the plate.
c) Wash the plate and add TMB or other colorimetric HRP substrate.
d) Stop the substrate reaction by adding diluted acid.
e) To remove background noise prior to statistical analysis, absorbance (OD) is calculated by substracting the value at 450 nm with the value at 630 nm. The OD Value reflects the amount of antibody bound.
Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Mouse IgG in 1:100 mouse serum. Detection was performed using HRP-Anti-mouse IgG antibody with sensitivity of 20 ng/mL (QC tested).
Price(USD) : $580.00
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