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This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 86.3 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM Nacl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mink ACE2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of SARS-CoV-2 S protein, His Tag, Super stable trimer (Cat. No. AC2-M52H3) is more than 85% and the molecular weight of this protein is around 175-195 kDa verified by SEC-MALS.
Mink ACE2, His Tag (Cat. No. AC2-M52H3) immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD (Y453F), His Tag (Cat. No. SPD-C52Hk) with an affinity constant of 0.178 μM as determined in a SPR assay (Biacore T200) (QC tested).
Mink ACE2, His Tag (Cat. No. AC2-M52H3) immobilized on CM5 Chip can bind SARS-CoV-2 S protein RBD, His Tag (Cat. No. SPD-C52H3) with an affinity constant of 8.34 μM as determined in a SPR assay (Biacore T200) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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