0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Immune Checkpoint Proteins > CD40 > CD0-M82F3

Biotinylated Mouse CD40 / TNFRSF5 Protein, Fc,Avitag™ (MALS verified)

  • Synonym
    CD40,Bp50,CDW40,MGC9013,TNFRSF5,p50
  • Source
    Biotinylated Mouse CD40, Fc,Avitag (CD0-M82F3) is expressed from human 293 cells (HEK293). It contains AA Val 24 - Arg 193 (Accession # P27512-1).
    Predicted N-terminus: Val 24
  • Molecular Characterization
    Online(Val 24 - Arg 193) P27512-1

    This protein carries a human IgG1 Fc tag at the C-terminus, followed by a Avi tag (Avitag™).

    The protein has a calculated MW of 47.1 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Mouse CD40, Fc,Avitag (Cat. No. CD0-M82F3) SDS-PAGE gel

Biotinylated Mouse CD40, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Mouse CD40, Fc,Avitag (Cat. No. ) MALS images

The purity of Biotinylated Mouse CD40, Fc,Avitag (Cat. No. CD0-M82F3) was more than 95% and the molecular weight of this protein is around 105-115 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Mouse CD40, Fc,AvitagBiotinylated Mouse CD40, Fc,Avitag (Cat. No. CD0-M82F3) ELISA bioactivity

Immobilized Biotinylated Mouse CD40, Fc,Avitag (Cat. No. CD0-M82F3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse CD40 Ligand, His Tag (active trimer) (Cat. No. CDL-M5248) with a linear range of 10-78 ng/mL (QC tested).

  • Background
    Tumor necrosis factor receptor superfamily member 5 (TNFRSF5) is also known as CD40, is a member of the TNF receptor superfamily. The expression of CD40 is diverse. TNFRSF5 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 is the receptor for TNFSF5/CD40LG. Defects in CD40 are the cause of immunodeficiency with hyper-IgM type 3 (HIGM3).
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $350.00

Price(USD) : $1380.00

Promotion & Exhibitions



Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:17 Details
  • Latest Research Phase:Phase 2 Clinical

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop