0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Immune Checkpoint Proteins > B7-H7 > B77-C52H3

Cynomolgus B7-H7 / HHLA2 Protein, His Tag (MALS verified)

  • Synonym
    B7-H7,HHLA2,B7 Homolog 7
  • Source
    Cynomolgus B7-H7, His Tag (B77-C52H3) is expressed from human 293 cells (HEK293). It contains AA Ile 21 - Asn 345 (Accession # XP_005548285.1).
  • Molecular Characterization
    Online(Ile 21 - Asn 345) XP_005548285.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 39.2 kDa. The protein migrates as 60-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus B7-H7, His Tag (Cat. No. B77-C52H3) SDS-PAGE gel

Cynomolgus B7-H7, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Cynomolgus B7-H7, His Tag (Cat. No. ) MALS images

The purity of Cynomolgus B7-H7, His Tag (Cat. No. B77-C52H3) was more than 90% and the molecular weight of this protein is around 50-60 kDa verified by SEC-MALS.

Bioactivity-BLI
Human_FcRn_Heterodimer_Protein_Bli

Loaded Cynomolgus B7-H7, His Tag (Cat. No. B77-C52H3) on HIS1K Biosensor, can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with an affinity constant of 13.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

  • Background
    B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

Comments (0)

Order Details

Price(USD) : $300.00

Price(USD) : $1980.00

Promotion & Exhibitions



Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

Contact Us

+1  800-810-0816 (US)
+86 400-682-2521 (AP)

Questions & Comments

This web search service is supported by Google Inc.

totop