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SARS-CoV-2 Spike NTD Protein (T19R, G142D, EF156-157del, R158G), His Tag (MALS verified)

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S1D-C52Hh-100ug
$330.00
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S1D-C52Hh-1mg
$2,350.00
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S1D-C52Hh
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Synonyms

S1 protein NTD, Spike protein S1 NTD, BetaCoV S1-NTD

Source

SARS-CoV-2 Spike NTD, His Tag (S1D-C52Hh) is expressed from human 293 cells (HEK293). It contains AA Ser 13 - Leu 303 (Accession # QHD43416.1). The mutations (T19R, G142D, EF156-157del, R158G) were identified in the SARS-CoV-2 Delta variant (Pango lineage: B.1.617.2; GISAID clade: GK; Nextstrain clade: 21A/21I/21J).

Predicted N-terminus: Ser 13

Molecular Characterization

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 34.6 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Endotoxin

Less than 1.0 EU per μg by the LAL method / rFC method.

Purity

>90% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

Formulation

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

Contact us for customized product form or formulation.

Reconstitution

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

Shipping and Storage

This product is shipped at ambient temperature.

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:
  • -20°C to -70°C for 12 months in lyophilized state;
  • -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.