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Your Position: Home > Immune Checkpoint Proteins > 4-1BB Ligand > 41L-R52D3

Rat 4-1BB Ligand / TNFSF9 Protein, His,Flag Tag (MALS verified)

  • Synonym
    4-1BB Ligand,TNFSF9,CD137L
  • Source
    Rat 4-1BB Ligand His,Flag Tag (41L-R52D3) is expressed from human 293 cells (HEK293). It contains AA Pro 106 - Gly 308 (Accession # Q80WE6-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Pro 106 - Gly 308) Q80WE6-1

    This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

    The protein has a calculated MW of 72.3 kDa. The protein migrates as 95-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Rat 4-1BB Ligand His,Flag Tag (Cat. No. 41L-R52D3) SDS-PAGE gel

Rat 4-1BB Ligand His,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Rat 4-1BB Ligand His,Flag Tag (Cat. No. ) MALS images

The purity of Rat 4-1BB Ligand His,Flag Tag (Cat. No. 41L-R52D3) was more than 90% and the molecular weight of this protein is around 169-206 kDa verified by SEC-MALS.

Bioactivity-ELISA
Rat 4-1BB Ligand His,Flag TagRat 4-1BB Ligand His,Flag Tag (Cat. No. 41L-R52D3) ELISA bioactivity

Immobilized Mouse 4-1BB, Fc Tag (Cat. No. 41B-M5258) at 1 μg/mL (100 μL/well) can bind Rat 4-1BB Ligand His,Flag Tag (Cat. No. 41L-R52D3) with a linear range of 5-39 ng/mL (QC tested).

  • Background
    Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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