Rat 4-1BB Ligand / TNFSF9 Protein, His,Flag Tag (MALS verified)

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Cat. No. / Size
Price
Qty
41L-R52D3-100ug
$360.00
41L-R52D3-1mg
$2615.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    4-1BB Ligand, TNFSF9, CD137L

  • Source

    Rat 4-1BB Ligand His,Flag Tag (41L-R52D3) is expressed from human 293 cells (HEK293). It contains AA Pro 106 - Gly 308 (Accession # Q80WE6-1).

    Predicted N-terminus: His

  • Molecular Characterization

    This protein carries a polyhistidine tag at the N-terminus, followed by a flag tag.

    The protein has a calculated MW of 72.3 kDa. The protein migrates as 95-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    4-1BB Ligand SDS-PAGE

    Rat 4-1BB Ligand His,Flag Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    4-1BB Ligand SEC-MALS

    The purity of Rat 4-1BB Ligand His,Flag Tag (Cat. No. 41L-R52D3) is more than 95% and the molecular weight of this protein is around 169-206 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     4-1BB Ligand ELISA

    Immobilized Mouse 4-1BB, Fc Tag (Cat. No. 41B-M5258) at 1 μg/mL (100 μL/well) can bind Rat 4-1BB Ligand His,Flag Tag (Cat. No. 41L-R52D3) with a linear range of 5-39 ng/mL (QC tested).

    Protocol

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Background

Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.

Recent Advances

 
Drug Development Progress
  • English Name:

    Tumor necrosis factor ligand superfamily member 9

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    2 Details

  • Highest Development Stage:

    Phase 2 Clinical

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