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Your Position: Home > Immune Checkpoint Proteins > 4-1BB Ligand > 41L-C5254

Cynomolgus 4-1BB Ligand / TNFSF9 Protein, Fc Tag, active trimer (MALS verified)

  • Synonym
    4-1BB Ligand,TNFSF9,CD137L
  • Source
    Cynomolgus 4-1BB Ligand, Fc Tag (41L-C5254) is expressed from human 293 cells (HEK293). It contains AA Leu 67 - Glu 251 (Accession # XP_015296398.1) trimer design.
    Predicted N-terminus: Pro
  • Molecular Characterization
    Online(Leu 67 - Glu 251) XP_015296398.1

    This protein carries a human IgG1 Fc tag at the N-terminus.

    The protein has a calculated MW of 87.4 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by reduced SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Cynomolgus 4-1BB Ligand, Fc Tag (Cat. No. 41L-C5254) SDS-PAGE gel

Cynomolgus 4-1BB Ligand, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

SEC-MALS
Cynomolgus 4-1BB Ligand, Fc Tag (Cat. No. ) MALS images

The purity of Cynomolgus 4-1BB Ligand, Fc Tag (Cat. No. 41L-C5254) was more than 90% and the molecular weight of this protein is around 160-196 kDa verified by SEC-MALS.

Bioactivity-ELISA
Cynomolgus 4-1BB Ligand, Fc TagCynomolgus 4-1BB Ligand, Fc Tag (Cat. No. 41L-C5254) ELISA bioactivity

Immobilized Human 4-1BB, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. 41B-H5256) at 2 μg/mL (100 μL/well) can bind Cynomolgus 4-1BB Ligand, Fc Tag (Cat. No. 41L-C5254) with a linear range of 0.2-2 ng/mL (QC tested).

  • Background
    Tumor necrosis factor ligand superfamily member 9 (4-1BBL) is also known as 4-1BB ligand, CD137L or TNFSF9, which is a cytokine that binds to TNFRSF9. 4-1BBL is the high affinity ligand of 4-1BB. 4-1BBL induces the proliferation of activated peripheral blood T-cells. Also, 4-1BBL may have a role in activation-induced cell death (AICD). Furthermore, 4-1BBL may play a role in cognate interactions between T-cells and B-cells/macrophages. As for diseases, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $350.00

Price(USD) : $2310.00

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