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Your Position: Home > ADC > Coagulation factor VII > TF7-H52H5

Human Coagulation factor VII / F7 Protein, His Tag (active enzyme, MALS verified)

  • Synonym
    Coagulation factor VII,F7,SPCA,Proconvertin
  • Source
    Human Coagulation factor VII, His Tag (TF7-H52H5) is expressed from human 293 cells (HEK293). It contains AA Ala 61 - Pro 466 (Accession # P08709-1).
  • Molecular Characterization
    Online(Ala 61 - Pro 466) P08709-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 47.1 kDa. The protein migrates as 50-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris,150 mM NaCl, pH7.5. Normally Trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Coagulation factor VII, His Tag (Cat. No. TF7-H52H5) SDS-PAGE gel

Human Coagulation factor VII, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Human Coagulation factor VII, His Tag (Cat. No. ) MALS images

The purity of Human Coagulation factor VII, His Tag (Cat. No. TF7-H52H5) was more than 85% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

Bioactivity

Measured by its ability to cleave the fluorogenic peptide substrate Boc-VPR-AMC. The specific activity is >80 pmol/min/µg, as measured under the described conditions (QC tested).

  • Background
    Coagulation factor VII is also known as F7, SPCA and Proconvertin. Heterodimer of a light chain and a heavy chain linked by a disulfide bond. Initiates the extrinsic pathway of blood coagulation. Serine protease that circulates in the blood in a zymogen form. Factor VII is converted to factor VIIa by factor Xa, factor XIIa, factor IXa, or thrombin by minor proteolysis. In the presence of tissue factor and calcium ions, factor VIIa then converts factor X to factor Xa by limited proteolysis. Factor VIIa will also convert factor IX to factor IXa in the presence of tissue factor and calcium.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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