Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of CD3E and CD3D, has a calculated MW of 38.3 kDa (CD3E) and 35.8 kDa (CD3D). Subunit CD3E is fused with a human IgG1 Fc tag at the C-terminus and subunit CD3D is fused with a human IgG1 Fc tag at the C-terminus. As a result of glycosylation, the protein migrates as 45-60 kDa under reducing (R) condition, and 90-110 kDa under non-reducing (NR) condition (SDS-PAGE).
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally Trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) was more than 90% and the molecular weight of this protein is around 75-85 kDa verified by SEC-MALS.
Immobilized Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), Ultra-low endotoxin (Cat. No. CDE-M120a) with a linear range of 0.8-6 ng/mL (QC tested).
Immobilized Human CD3E&CD3D Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. CDD-H5255) at 1 μg/mL (100 μL/well) can bind UCHT1 with a linear range of 0.8-3 ng/mL (Routinely tested).
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