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Your Position: Home > Cytokines > TGF-beta 3 > TG3-H82E6

Biotinylated Human Latent TGF-beta 3 / Latent TGFB3 Protein, His,Avitag™

  • Synonym
    TGFB3,ARVD,TGF-beta3
  • Source
    Biotinylated Human Latent TGFB3, His,Avitag (TG3-H82E6) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Ser 412 (Accession # P10600-1).
    Predicted N-terminus: His
  • Molecular Characterization
    Online(Leu 24  - Ser 412) P10600-1

    This protein carries a polyhistidine tag at the N-terminus and an Avi tag at the C-terminus.

    The protein has a calculated MW of 48.4 kDa. The protein migrates as 40-45 kDa and 60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human Latent  TGFB3, His,Avitag (Cat. No. TG3-H82E6) SDS-PAGE gel

Biotinylated Human Latent TGFB3, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Biotinylated Human Latent  TGFB3, His,AvitagBiotinylated Human Latent  TGFB3, His,Avitag (Cat. No. TG3-H82E6) ELISA bioactivity

Immobilized Human TGFBR2, Fc Tag (Cat. No. TG2-H5252) at 5 μg/mL (100 μL/well) can bind Biotinylated Human Latent TGFB3, His,Avitag (Cat. No. TG3-H82E6) with a linear range of 0.3-20 ng/mL (QC tested).

  • Background
    Transforming growth factor beta 3 ( TGFB3) is also known as TGF-β3, is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including regulates embryogenesis and cell differentiation and is required in various processes such as secondary palate development. TGF-beta 3 is similar with TGF-beta 1 and ­-beta 2, act as cellular switches to regulate immune function, cell proliferation, and epithelial­-mesenchymal transition. TGF-beta-3 is released from LAP by integrins: integrin-binding results in distortion of the LAP chain and subsequent release of the active TGF-beta-3. Once activated following release of LAP, TGF-beta-3 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $650.00

Price(USD) : $1800.00

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  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 1 Clinical

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