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Your Position: Home > Antibody > Rituximab > RIB-Y35c

Anti-Rituximab Antibody (MALS verified)

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  • Source
    Anti-Rituximab Antibody (RIB-Y35c) are expressed from human 293 cells (HEK293).
  • Isotype
    Mouse IgG1/kappa
  • Specificity
    Recognizes Rituximab specifically, no cross reactivity with other humanized antibodies.
  • Application
    This antibody can be paired with other CD20 antibodies to detect CD20 in sandwich ELISA or LFA assay.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 12 months under sterile conditions after reconstitution.
Rituximab SDS-PAGE

Anti-Rituximab Antibody on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

Rituximab MALS images

The purity of Anti-Rituximab Antibody (Cat. No. RIB-Y35c) is more than 90% and the molecular weight of this protein is around 135-155kDa verified by SEC-MALS.

 Rituximab ELISA

Immobilized Rituximab at 1 μg/mL (100 μL/well) can bind Anti-Rituximab Antibody (Cat. No. RIB-Y35c) with a linear range of 0.2-6 ng/mL (QC tested).

 Rituximab ELISA

Immobilized Rituximab at 2 μg/mL, add increasing concentrations of Anti-Rituximab Antibody (Cat. No. RIB-Y35c) in the 10% human serum and then add Anti-CD20, Human IgG1 at 5 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 100 ng/mL (QC tested).

  • Background
    Rituxan is a genetically engineered chimeric murine/human monoclonal antibody directed against the CD20 antigen found on the surface of normal and malignant B lymphocytes. The antibody is an IgG1 kappa immunoglobulin containing murine light- and heavy-chain variable region sequences and human constant region sequences. Rituximab is composed of two heavy chains of 451 amino acids and two light chains of 213 amino acids, Anti-Rituximab Antibodies (RIB-Y35c) is the monoclonal antibody of Rituximab expressed with human HEK293 cells.
  • Clinical and Translational Updates

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