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Biotinylated Cynomolgus TSLP Protein, His,Avitag™ (MALS verified)

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Cat. No. / Size
Price
Qty
TSP-C82E3-25ug
$360.00
TSP-C82E3-200ug
$1565.00
ETA of in-stock products:2 business days
Sub-Total$ 0

Product Details

  • Synonyms

    TSLP

  • Source

    Biotinylated Cynomolgus TSLP, His,Avitag (TSP-C82E3) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # A0A7N9CAT7-1).

    Predicted N-terminus: Tyr 29

    Request for sequence
  • Molecular Characterization

    TSLP Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 18.8 kDa. The protein migrates as 27 kDa and 29 kDa under non-reducing (NR) condition (SDS-PAGE).

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    TSLP SDS-PAGE

    Biotinylated Cynomolgus TSLP, His,Avitag on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    TSLP SEC-MALS

    The purity of Biotinylated Cynomolgus TSLP, His,Avitag (Cat. No. TSP-C82E3) is more than 90% and the molecular weight of this protein is around 20-35 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     TSLP ELISA

    Immobilized Human TSLP R, Fc Tag (Cat. No. TSR-H525a) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus TSLP, His,Avitag (Cat. No. TSP-C82E3) with a linear range of 0.1-2 ng/mL (QC tested).

    Protocol
  •  TSLP ELISA

    Immobilized Monoclonal Anti-Human TSLP Antibody, Human IgG2 at 2 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus TSLP, His,Avitag (Cat. No. TSP-C82E3) with a linear range of 0.1-1 ng/mL (Routinely tested).

    Protocol

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Background

Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.

Recent Advances

 
Drug Development Progress
  • English Name:

    Thymic stromal lymphopoietin

  • Category:

  • Approved Drugs:

    1 Details

  • Drugs in Clinical Trials:

    29 Details

  • Highest Development Stage:

    Approved

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