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This protein carries a human IgG1 Fc tag at the C-terminus
The protein has a calculated MW of 59.5 kDa. The protein migrates as 90-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
FITC
Excitation source: 488 nm spectral line, argon-ion laser
Excitation Wavelength: 488 nm
Emission Wavelength: 535 nm
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
FITC-Labeled Human IL-3 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Anti-IL-3 R alpha Antibody, Human IgG1 at 1 μg/mL (100 μL/well) can bind FITC-Labeled Human IL-3 R alpha, Fc Tag (Cat. No. ILA-HF253) with a linear range of 0.078-0.625 μg/mL (QC tested).
2e5 of anti-IL-3 R alpha CAR-293 cells were stained with 100 μL of 10 μg/mL of FITC-Labeled Human IL-3 R alpha, Fc Tag (Cat. No. ILA-HF253) and negative control protein respectively and analyzed with FACS (QC tested).
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