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Your Position: Home > Receptor Tyrosine Kinases > EGF R > EGR-H5259

Human EGF R Protein, Llama IgG2b Fc Tag, low endotoxin

  • Synonym
    EGFR,ERBB,ERBB1,HER1,PIG61,mENA
  • Source
    Human EGF R, Llama IgG2b Fc Tag (EGR-H5259) is expressed from human 293 cells (HEK293). It contains AA Leu 25 - Ser 645 (Accession # P00533-1).
    Predicted N-terminus: Leu 25
  • Molecular Characterization
    Online(Leu 25 - Ser 645) P00533-1

    This protein carries a llama IgG2b Fc tag at the C-terminus.

    The protein has a calculated MW of 96.4 kDa. The protein migrates as 115-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method.
  • Purity

    >95% as determined by reduced SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human EGF R, Llama IgG2b Fc Tag (Cat. No. EGR-H5259) SDS-PAGE gel

Human EGF R, Llama IgG2b Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human EGF R, Llama IgG2b Fc TagHuman EGF R, Llama IgG2b Fc Tag (Cat. No. EGR-H5259) ELISA bioactivity

Immobilized Human EGF, His Tag (Cat. No. EGF-H52H3) at 5 μg/mL (100 μL/well) can bind Human EGF R, Llama IgG2b Fc Tag (Cat. No. EGR-H5259) with a linear range of 0.2-5 ng/mL (QC tested).

Human EGF R, Llama IgG2b Fc TagHuman EGF R, Llama IgG2b Fc Tag (Cat. No. EGR-H5259) ELISA bioactivity

Immobilized Human EGF R, Llama IgG2b Fc Tag (Cat. No. EGR-H5259) at 2 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.3-5 ng/mL (Routinely tested).

Please note that there may be a cross-reaction between anti-human IgG Fc antibodies and llama IgG Fc tag, also between anti-llama IgG Fc antibodies and human IgG Fc tag.
  • Background
    The epidermal growth factor receptor (EGFR; ErbB-1; HER1) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $300.00

Price(USD) : $1980.00

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