This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 23.7 kDa. The protein migrates as 39-51 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CD16a (F176), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human CD16a (F176), His Tag (Cat. No. CDA-H5220) was more than 90% and the molecular weight of this protein is around 30-40 kDa verified by SEC-MALS.
Immobilized Human CD16a (F176), His Tag (Cat. No. CDA-H5220) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 2.70 μM as determined in a SPR assay (Biacore 8K) (QC tested).
Loaded Human CD16a (F176), His Tag (Cat. No. CDA-H5220) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1.72 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Herceptin on Protein A Biosensor, can bind Human CD16a (F176), His Tag (Cat. No. CDA-H5220) with an affinity constant of 0.47 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Herceptin on FAB2G Biosensor, can bind Human CD16a (F176), His Tag (Cat. No. CDA-H5220) with an affinity constant of 0.51 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Zhao Y, et al
Journal: Cancer Biol Ther 2019
Authors: Simonov V, et al
Journal: Biologicals 2019
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