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Your Position: Home > 2019-nCoV proteins > Spike RBD > EP-107

SARS-CoV-2 (WT) Inhibitor Screening Kit (Spike RBD)

For research use only.
Materials Provided
EP107-C01Pre-coated Human ACE2 Microplate1 plate
EP107-C02SARS-CoV-2 Inhibitor10 μg
EP107-C03HRP-SARS-CoV-2 Spike RBD(WT)15 μg
EP107-C0410xWashing Buffer50 mL
EP107-C05Dilution Buffer50 mL
EP107-C06Substrate Solution12 mL
EP107-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) poses continual threat to human health due to rapid transmission worldwide. The deleloped of effcient treatment agaginst SARS-CoV-2 is an emergent need for global pubic heath,and it is necessary to develop SARS-CoV-2 inhibitors, such as vaccines、 therapeutic antibodies and small molecule compounds against SARS-CoV-2. Therefore, it is helpful to develop the SARS-CoV-2 (WT) Inhibitor Screening Kit (Spike RBD) to test SARS-CoV-2 inhibitors.
  • Application
    This kit is developed for screening inhibitors of SARS-CoV-2(WT).
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.

  • Assay Principles
    This assay kit is used to measure the levels of Anti-SARS-CoV-2 neutralizing antibody through a competitive ELISA. The microplate in the kit has been pre-coated with Human ACE2 protein. First samples are added to the wells followed by addition of HRP-SARS-CoV-2 Spike RBD. After incubation, the wells are washed and substrate is added to the wells. The reaction is terminated by the addition of stop solution and the intensity of color is measured at 450 nm. The presence of neutralizing antibodies in samples will compete with ACE2 for HRP-SARS-CoV-2 Spike RBD binding. The intensity of assay signal decrease proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.

    Your experiment will include 5 simple steps:

    a)All reagents were returned to room temperature(20℃-25℃) before use.

    b)Make series the tested sample and control with ditlution buffer,HRP-SARS-CoV-2 RBD diluted with dilution buffer.

    c) Add the diluted sample ,Control and the HRP-SARS-CoV-2 RBD add the plate respectively.

    d) Wash the plate and add TMB or other colorimetric HRP substrate. e) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
SARS-CoV-2 (WT) Inhibitor Screening Kit (Spike RBD)SARS-CoV-2 (WT) Inhibitor Screening Kit (Spike RBD) (Cat. No. EP-107) ELISA bioactivity

Inhibition of HRP-SARS-CoV-2 Spike RBD(WT):SARS-CoV-2 Spike RBD Binding by SARS-CoV-2 inhibitor.
Serial dilutions of SARS-CoV-2 inhibitor (Catalog # EP107-C02) (1:1 serial dilutions, from 5.0ug/mL to 0.0098ug/mL) and loaded onto the plate coated by human ACE2 in the presence of HRP-SARS-CoV-2 Spike RBD(WT).Background was subtracted from data points prior to log transformation and curve fitting.

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Price(USD) : $600.00

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