This protein carries a polyhistidine tag at the C-terminus. This protein undergoes autocatalytic cleavage to release the pro-peptide and mature chain. The pro-peptide and mature chain are associated through non‑covalent interactions and with a calculated MW of 13.9 kDa and 58.2 kDa respectively. The protein migrates as 17 kDa and 65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Mouse PCSK9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human LDL R, Fc Tag (Cat. No. LDR-H5254) at 5 μg/mL (100 μL/well) can bind Mouse PCSK9, His Tag (Cat. No. PC9-M5228) with a linear range of 0.078-0.313 μg/mL (QC tested).
Immobilized Mouse PCSK9, His Tag (Cat. No. PC9-M5228) at 1 μg/mL (100 μL/well) on Monoclonal Anti-His Tag Antibody, Mouse IgG1 (AY63) (Cat. No. HIS-AY63) precoated (0.1 μg/well) plate can bind Evolocumab with a linear range of 2-31 ng/mL (Routinely tested).
Loaded Human LDL R, Fc Tag (Cat. No. LDR-H5254) on Protein A Biosensor, can bind Mouse PCSK9, His Tag (Cat. No. PC9-M5228) with an affinity constant of 2.28 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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