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Your Position: Home > Protein > B7-H7 > B77-H82E3

Biotinylated Human B7-H7 / HHLA2 Protein, His,Avitag™ (MALS verified)

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  • Synonym
    B7-H7,HHLA2,B7 Homolog 7
  • Source
    Biotinylated Human B7-H7, His,Avitag™ (B77-H82E3) is expressed from human 293 cells (HEK293). It contains AA Ile 23 - Asn 344 (Accession # Q9UM44-1).
    Predicted N-terminus: Ile 23
  • Molecular Characterization
    Online(Ile 23 - Asn 344) Q9UM44-1

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 40.7 kDa. The protein migrates as 50-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Biotinylation
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Biotin:Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Biotinylated Human B7-H7, His,Avitag™ (Cat. No. B77-H82E3) SDS-PAGE gel

Biotinylated Human B7-H7, His,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Biotinylated Human B7-H7, His,Avitag™ (Cat. No. ) MALS images

The purity of Biotinylated Human B7-H7, His,Avitag™ (Cat. No. B77-H82E3) is more than 90% and the molecular weight of this protein is around 48-73 kDa verified by SEC-MALS.

Bioactivity-ELISA
Biotinylated Human B7-H7, His,Avitag™Biotinylated Human B7-H7, His,Avitag™ (Cat. No. B77-H82E3) ELISA bioactivity

Immobilized Biotinylated Human B7-H7, His,Avitag (Cat. No. B77-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CD28H, Fc Tag (Cat. No. CDH-H5251) with a linear range of 0.1-3 ng/mL (QC tested).

Biotinylated Human B7-H7, His,Avitag™Biotinylated Human B7-H7, His,Avitag™ (Cat. No. B77-H82E3) ELISA bioactivity

Immobilized Human CD28H, Fc Tag (Cat. No. CDH-H5251) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H7, His,Avitag (Cat. No. B77-H82E3) with a linear range of 0.039-1.25 μg/mL (Routinely tested).

  • Background
    B7-H7 (HHLA2) is a newly identified B7 family member that regulates human T-cell functions. B7-H7 was previously known as human endogenous retrovirus-H long terminal repeat associating 2 (HHLA2) with unidentified function. Recently, B7-H7 has been identified as a specific ligand for human CD28H. The B7-H7-CD28H pathway strongly promoted CD4+ T-cell proliferation and cytokine production via an AKT-dependent signaling cascade in the presence of TCR signaling, suggesting B7-H7 comprises a new co-stimulatory pathway. The first IgV domain of B7-H7, which presumably binds to a putative receptor, shows the highest homology to other B7 family members.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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