This protein carries a polyhistidine tag at the N-terminus
The protein has a calculated MW of 23.0 kDa. The protein migrates as 25-27 kDa under reducing (R) condition (SDS-PAGE).
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in 50mM Tris,500mM NaCl pH7.3 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human KRAS (2-185,G12D), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human KRAS (2-185,G12D), His Tag (Cat. No. KRS-H51H4) is more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.
GTPase-Glo test kras activity,the GTP is ≤95%, as measured under the described conditions (QC tested).
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
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