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Your Position: Home > 2019-nCoV proteins > Spike protein > RAS-T025

Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)

For research use only.
Materials Provided
IDComponentsSize
RAS025-C01Pre-coated SARS-CoV-2 Spike Protein Microplate1 plate
RAS025-C02Positive Control100 μL
RAS025-C03Negative Control100 μL
RAS025-C04HRP-Anti-Human IgG200 μL
RAS025-C0510xWashing Buffer50 mL
RAS025-C06Dilution Buffer50 mL
RAS025-C07Substrate Solution12 mL
RAS025-C08Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for titer measurement of Anti-SARS-CoV-2 Antibody IgG (Spike Trimer) in human serum.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit should be stored at 2°C to 8°C. The shelf life is 1 year from the date of receipt. The opened kit should be stored at 2°C to 8°C. The shelf life is 1 month from the date of opening.

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgG in serum by SARS-CoV-2 Spike Protein. The kit consists of Pre-coated SARS-CoV-2 Spike Protein Microplate, an Positive Control, an Negative Control,an HRP-Anti-Human IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps: a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Dilution Buffer.

    b) Add a diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data
Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer)Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike Trimer) (Cat. No. RAS-T025) ELISA bioactivity

Detection of Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike Protein at 2 μg/mL (100 μL/well) can bind monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:400 human serum. Detection was performed using HRP- Anti-human IgG antibody with sensitivity of 98 ng/mL (QC tested).

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Price(USD) : $580.00

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  • Latest Research Phase:Approved

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