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Your Position: Home > IgG kits > Spike RBD > RAS-T024

Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay Kit (Spike RBD)

For research use only.
IDComponentsSize
RAS024-C01Pre-coated SARS-CoV-2 Spike RBD Microplate1 plate
RAS024-C02Positive Control100 μL
RAS024-C03Negative Control100 μL
RAS024-C04HRP-Anti-Human IgG200 μL
RAS024-C0510xWashing Buffer50 mL
RAS024-C06Dilution Buffer50 mL
RAS024-C07Substrate Solution12 mL
RAS024-C08Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective Assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for titer measurement of Anti-SARS-CoV-2 Antibody IgG (Spike RBD) in human serum.

    It is for research use only.

  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage & Shipping
    The unopened kit is stable for 12 months from the date of manufacture if stored at 2°C to 8°C.

    The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

    The kit shipped at room temperature that had been validated. Please contact us if you need blue ice shipping, but additional freight may be followed.

    Below is the Shipping Statement for Kit Products.

    Shipping Statement

  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of Anti-SARS-CoV-2 antibodies in serum by SARS-CoV-2 Spike RBD. The kit consists of Pre-coated SARS-CoV-2 Spike RBD Microplate, an Positive Control, an Negative Control,an HRP-Anti-Human IgG secondary antibody and related buffer.

    Your experiment will include 4 simple steps:

    a) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.

    b) Add diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.

    c) Wash the plate and add TMB or other colorimetric HRP substrate.

    d) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

Typical Data Please refer to DS document for The assay protocol.
 Spike RBD ELISA

Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay.
Immobilized SARS-CoV-2 Spike RBD at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:400 human serum. Detection was performed using HRP-Anti-human IgG antibody with sensitivity of 98 ng/mL (QC tested).

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Price(USD) : $580.00

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