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Your Position: Home > All Other Proteins > Semaphorin 3A > SEA-H5257

Human Semaphorin 3A / SEMA3A Protein, Fc Tag

  • Synonym
    Semaphorin 3A,(semaphorin) 3A,coll-1,collapsin 1,Hsema-I,Hsema-III,MGC133243,sema domain,immunoglobulin domain (Ig),short basic domain,secreted,Sema III,SEMA1, Sema3A,SEMAD,SEMAIII,SEMAL,Semaphorin 3A,semaphorin D,Semaphorin III,semaphorin L,semaphorin-3A
  • Source
    Human Semaphorin 3A, Fc Tag (SEA-H5257) is expressed from human 293 cells (HEK293). It contains AA Lys 26 - Val 771 (Accession # Q14563-1(R552A, R555A)).
  • Molecular Characterization
    Online(Lys 26 - Val 771) Q14563-1 (R552A, R555A)

    This protein carries a human IgG1 Fc tag at the N-terminus. The protein has a calculated MW of 112.4 kDa. Besides Furin cleavage site (Arg 555 - Gln 556), the protein also has an unknown protease cleavage site at Leu 709 - Ile 710. The protein migrates as 100 kDa and 116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5257) SDS-PAGE gel

Human Semaphorin 3A, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human Semaphorin 3A, Fc TagHuman Semaphorin 3A, Fc Tag (Cat. No. SEA-H5257) ELISA bioactivity

Immobilized Biotinylated Human Neuropilin-1, His,Avitag (Cat. No. NR1-H82E3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Semaphorin 3A, Fc Tag (Cat. No. SEA-H5257) with a linear range of 5-156 ng/mL (QC tested).

  • Background
    Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $400.00

Price(USD) : $2400.00

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