Product Details
Synonyms
IL-2 R beta & IL-2 R gamma, IL-2RB & IL-2RG
Source
Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (ILG-M5254) is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Glu 240 (IL-2RB) & Trp 23 - Ala 263 (IL-2RG) (Accession # P16297-1 (IL-2RB) & P34902-1 (IL-2RG)).
Predicted N-terminus: Ala 27 (IL-2RB) & Trp 23 (IL-2RG)
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag is produced by co-expression of IL-2RB and IL-2RG, has a calculated MW of 51.5 kDa (IL-2RB) and 54.5 kDa (IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-2RG is fused with a human IgG1 Fc tag at the C-terminus. The protein migrates as 65-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>95% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
SEC-MALS

The purity of Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILG-M5254) is more than 85% and the molecular weight of this protein is around 138-168 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Biotinylated Human IL-2, His,Avitag (Cat. No. IL2-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Mouse IL-2RB&IL-2RG Heterodimer Protein, Fc Tag&Fc Tag (Cat. No. ILG-M5254) with a linear range of 0.008-0.25 μg/mL (QC tested).
Protocol
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Background
Recent Advances
- English Name:
Interleukin-2 receptor subunit gamma
- Category:
- Approved Drugs:
0 Details
- Drugs in Clinical Trials:
14 Details
- Highest Development Stage:
Phase 2 Clinical
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