|Items||Size （2mg）||Size（5mg X 2）|
|Particle size||2 μm||2 μm|
|Physical appearance||Powder mixture||Powder mixture|
|Amount of Coupled Protein||>940 pmol (15.5 μg) CD47 protein/mg beads||>940 pmol (15.5 μg) CD47 protein/mg beads|
|Binding Capacity||>250 pmol (45 μg) Anti-CD47 antibody/mg beads||>250 pmol (45 μg) Anti-CD47 antibody/mg beads|
|Formulation||PBS, pH7.4, with 10% Trehalose||PBS, pH7.4, with 10% Trehalose|
|Reconstitution||2 mL ultrapure water (1 mg beads/mL)||5 mL ultrapure water (1 mg beads/mL)|
Upon receipt, please store the beads at -20℃ for 1 year in lyophilized state.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
Immobilized 15.8 μg CD47/1mg beads can bind the Anti CD47 Ab (Cat. No. CD7-M12) with an EC50 of 0.5720 μg/mL (QC tested).
Price(USD) : $800.00
Price(USD) : $2500.00
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