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Your Position: Home > Protein-coupled magnetic beads > CD47 > MBS-K023

Human CD47-coupled Magnetic Beads

Materials Provided
ItemsSize (2mg)Size(5mg X 2)
Particle size2 μm2 μm
Physical appearancePowder mixturePowder mixture
Amount of Coupled Protein>940 pmol (15.5 μg) CD47 protein/mg beads>940 pmol (15.5 μg) CD47 protein/mg beads
Binding Capacity>250 pmol (45 μg) Anti-CD47 antibody/mg beads>250 pmol (45 μg) Anti-CD47 antibody/mg beads
FormulationPBS, pH7.4, with 10% TrehalosePBS, pH7.4, with 10% Trehalose
Reconstitution2 mL ultrapure water (1 mg beads/mL)5 mL ultrapure water (1 mg beads/mL)
  • Background
    The Human CD47-coupled Magnetic Beads is produced by coupling biotinylated CD47 protein to streptavidin-conjugated magnetic beads. Because Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10-14 mol/L, the biotinylated protein can bind to the SA beads irreversibly. The pre-coupled beads are ready to use for capturing anti-CD47 antibody from your sample with high specificity.
  • Application
    This product is intended for immunocapture, biopanning and flow cytometry.
  • Reconstitution
    See Certificate of Analysis (CoA) for detailed instruction.
  • Storage

    Upon receipt, please store the beads at -20℃ for 1 year in lyophilized state.

    Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.

  • Assay Principles
    The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.

    Antibody Purification:

    1. Resuspend the lyophilized beads by adding the buffer of choice.

    2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.

    3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.

    4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.

Typical Data
Human CD47-coupled Magnetic BeadsHuman CD47-coupled Magnetic Beads (Cat. No. MBS-K023) ELISA bioactivity

Immobilized 15.8 μg CD47/1mg beads can bind the Anti CD47 Ab (Cat. No. CD7-M12) with an EC50 of 0.5720 μg/mL (QC tested).

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Price(USD) : $800.00

Price(USD) : $2500.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:20 Details
  • Latest Research Phase:NDA/BLA

Datasheet & Documentation

Request for DMF
DMF (Drug Master File)

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