Human FOLR3 Protein, His Tag (MALS verified)

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Cat. No. / Size
Price
Qty
FO3-H52H3-100ug
$310.00
FO3-H52H3-1mg
$2245.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    folate receptor 3 (gamma), Folate receptor 3, folate receptor gamma, FOLR3, FR-G, FR-gamma, gamma-Hfr,

  • Source

    Human FOLR3, His Tag (FO3-H52H3) is expressed from human 293 cells (HEK293). It contains AA Gln 23 - Ser 245 (Accession # P41439-1).

    Predicted N-terminus: Gln 23

    Request for sequence
  • Molecular Characterization

    FOLR3 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 27.4 kDa. The protein migrates as 33-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >90% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    FOLR3 SDS-PAGE

    Human FOLR3, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

  • SEC-MALS

    FOLR3 SEC-MALS

    The purity of Human FOLR3, His Tag (Cat. No. FO3-H52H3) is more than 95% and the molecular weight of this protein is around 28-42 kDa verified by SEC-MALS.

    Report
  • Bioactivity-SPR

     FOLR3 SPR

    Folic acid-BSA immobilized on CM5 Chip can bind Human FOLR3, His Tag (Cat. No. FO3-H52H3) with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

    Protocol

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Background

FOLR3, also know as folate receptor gamma, is a member of FRs. Folic acid and its reduced derivatives are transported via two widely expressed transporters, the reduced folate carrier (RFC) and the proton-coupled folate transporter (PCFT), and via a family of glycosyl-phosphatidylinositol (GPI)-anchored receptors with limited expression profiles known as folate receptors (FRs).Folate receptors (FR) are high affinity receptors that transport folate via endocytosis. Human FR exists in three isoforms, alpha and beta equipped with GPI anchors, and gamma not possessing a GPI anchor.

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